Dataset Information

Exposure of a bovine rumen microbiome model to glyphosate

ABSTRACT: We evaluated the effects of pure glyphosate and the formulations Durano TF and Roundup® LB plus in different concentrations on rumen microbiota bioreactor model using the Rumen Simulation Technique (RUSITEC). Application of the compounds in three concentrations (0.1 mg/L, 1.0 mg/L or 10 mg/L, n = 4 each).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: Sven Haange

LAB HEAD: Nico Jehmlich

PROVIDER: PXD032166 | Pride | 2022-05-08

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	180501_LAM_V3-A_180502111740.msf	Msf
	180501_LAM_V3-A_180502111740.raw	Raw
	180501_LAM_V3-B_180502182314.raw	Raw
	180501_LAM_V3-C_180502215607.raw	Raw
	180501_LAM_V3-D_180503012856.raw	Raw

Items per page:

1 - 5 of 98

Similar Datasets

Project description:In vitro toxicology approaches have evolved, from a focus on the molecular changes within a cell to understanding of toxicity-related mechanisms that simulate the in vivo environment. The recent development of three dimensional (3-D) organotypic nasal epithelial culture models offer a physiologically robust system for studying the effects of exposure through inhalation. Exposure to cigarette smoke (CS) is associated with nasal inflammation; thus the nasal epithelium is relevant for evaluating the pathophysiological impact of CS exposure. The present study investigated further the relevancy and application of in vitro human 3-D nasal epithelial culture models for toxicological assessment of inhalation exposure. The biological impact was assessed following exposure to aerosol generated from a candidate modified risk tobacco product (MRTP), the Tobacco Heating System (THS) 2.2, as compared with smoke generated from reference cigarette 3R4F using an in vitro human 3-D nasal epithelial cultures. A series of experimental repetitions where multiple doses of the aerosol and smoke were applied, were conducted to obtain reproducible measurements and reliable observations to understand the cellular/molecular changes that occur following exposure. Aligned with the 3Rs Strategy and the Vision-and-Strategy of the Toxicity Testing in the 21st Century, this study implemented a systems toxicology approach and found that for all tested concentrations, the impact of 3R4F smoke was considerably greater than that of THS2.2 aerosol in terms of cytotoxicity levels, alterations in the tissue morphology, secretion of pro-inflammatory mediators, impaired ciliary function, and increased perturbed transcriptomes and miRNA expression profiles. In addition, to evaluate further the possible adverse effects of THS2.2 aerosol, a dose range assessment was conducted. A broader range of THS2.2 concentrations were exposed to the nasal cultures. Various dilutions of THS2.2 were applied to the cultures using the Vitrocellï¾® 24/48 exposure system, corresponding to the concentrations of nicotine between 0.15 mg/L and 1.79 mg/L

Dataset Information

Exposure of a bovine rumen microbiome model to glyphosate

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets