Project description:In this project we investigated the effect of YAP1 mutations on the interactome. We investigate the effect of S61A, S109A, S127A, S138A, S143A, S367A, 5SA, delta WW1, delta WW2, delta WW1/WW2
Project description:In this project we investigated the effect of YAP1 mutations on the interactome. We investigate the effect of S61A, S109A, S127A, S138A, S143A, S367A, 5SA.
Project description:Determination of the interaction partners of endogenous UBASH3B in A549 cells (PRM) and validation of the interactome after purification of Strep C- and N-terminally tagged UBASH3B in HEK293 FlpIn cell line (DDA).
Project description:The treatment of colorectal cancer (CRC) is an unmet medical need in absence of early diagnosis. Here, upon characterizing cancer-specific transposable element-driven transpochimeric gene transcripts (TcGTs) produced by this tumor, we found that expression of the hominid-restricted retrogene POU5F1B through aberrant activation of a primate-specific endogenous retroviral promoter is a strong negative prognostic CRC biomarker. Correlating this observation, we could demonstrate that POU5F1B fosters the proliferation and metastatic potential of CRC cells. We further determined that POU5F1B, in spite of its phylogenetic relationship with the POU5F1/OCT4 transcription factor, is a membrane-enriched protein that associates with protein kinases and known targets or interactors as well as with cytoskeleton-related molecules, and induces intracellular signaling events and the release of trans-acting factors involved in cell growth and cell adhesion. As POU5F1B is an apparently non-essential gene only lowly expressed in normal tissues, and as POU5F1B-containing TcGTs are detected in other tumors besides CRC, our data provide interesting leads for the development of cancer therapies.