Dataset Information

Complete metabolic reconstruction of the microbiome of the model sponge Ianthella basta

ABSTRACT: Marine sponges are essential for coral reefs to thrive and harbour a diverse microbiome that is thought to contribute to host health. Although the overall function of sponge symbionts has been increasingly described, in-depth characterisation of each taxa remains challenging, with many sponge species hosting up to 3,000 distinct microbial species. Recently, the sponge Ianthella basta has emerged as a model organism for symbiosis research, hosting only three dominant symbionts: a Thaumarchaeotum, a Gammaproteobacterium, and an Alphaproteobacterium and a range of other minor taxa. Here, we retrieved metagenome assembled genomes (MAGs) for >90% of I. basta’s microbial community which allowed us to make a complete metabolic reconstruction of the sponge’s microbiome, identifying metabolic complementarity between microbes, as well as the importance of symbionts present in low abundance. We also mined the metagenomes for putative viral sequences, highlighting the contribution of viruses to the overall metabolism of the sponge, and complement this data with metaproteomic sequencing to identify active metabolic pathways in both prokaryotes and viruses. This data now allows us to use I. basta as a model organism for studying host-microbe interactions and provides a basis for future (genomic) manipulative experiments.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Ianthella Basta

SUBMITTER: Nico Jehmlich

LAB HEAD: Nico Jehmlich

PROVIDER: PXD032278 | Pride | 2023-02-24

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	2021_09_24_1_Sponge2_Rep4.raw	Raw
	2021_09_24_2_Sponge2_Rep2.raw	Raw
	2021_09_24_3_Sponge3_Rep2.raw	Raw
	2021_09_24_4_Sponge3_Rep3.raw	Raw
	2021_09_24_5_Sponge1_Rep1.raw	Raw

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Publications

Metabolic reconstruction of the near complete microbiome of the model sponge Ianthella basta.

Engelberts Joan Pamela JP Robbins Steven J SJ Herbold Craig W CW Moeller Florian U FU Jehmlich Nico N Laffy Patrick W PW Wagner Michael M Webster Nicole S NS

Environmental microbiology 20221223 3

Many marine sponges host highly diverse microbiomes that contribute to various aspects of host health. Although the putative function of individual groups of sponge symbionts has been increasingly described, the extreme diversity has generally precluded in-depth characterization of entire microbiomes, including identification of syntrophic partnerships. The Indo-Pacific sponge Ianthella basta is emerging as a model organism for symbiosis research, hosting only three dominant symbionts: a Thaumar ...[more]

PMID: 36480164

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