Dataset Information

Discovery and validation of serum glycoprotein biomarker panel for detection of high grade serous ovarian cancer

ABSTRACT: We aimed to discover and validate a panel of serum biomarkers for high grade serous ovarian cancer (HGSOC) using our lectin-magnetic bead array-coupled proteomics platform. Serum from age-matched women with HGSOC, benign tumours or healthy controls were analysed in discovery (UKCTOCS, n=30 and UKOPS, n=30) and validation (Australian Ovarian Cancer Study, n=95) cohorts using shotgun and targeted proteomics, respectively. A 7-lectin discovery screen shortlisted 60 candidate proteins and 3 lectins for validation, which revealed elevated levels of AAL, SNA or STL-binding FIBB, CO9, ITIH3, HPT, A1AT, AACT in HGSOC, while IBP3, A2MG, PON1, CHLE and ALS were reduced. Multimarker panels were developed using generalized regression with lasso estimation and leave-one-out cross-validation. The best performing panel comprised of 13 peptides from Solanum Tuberosum lectin (STL)-binding proteins with 96.3% area under the receiver operating curve, 97.7% specificity and 78.6% sensitivity for distinguishing HGSOC from benign and healthy groups. The peptides robust in cross-validations were from IBP3, KNG1, CO9, THRB, HPTR, HPT, FINC, FA10, GELS. The validated serum biomarkers show promise for early detection of HGSOC and should be further evaluated.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Thomas Stoll

LAB HEAD: Michelle M. Hill

PROVIDER: PXD032299 | Pride | 2023-10-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20191206_OvCa_Lemba_AAL_10.wiff	Wiff
	20191206_OvCa_Lemba_AAL_10.wiff.scan	Wiff
	20191206_OvCa_Lemba_AAL_11.wiff	Wiff
	20191206_OvCa_Lemba_AAL_11.wiff.scan	Wiff
	20191206_OvCa_Lemba_AAL_12.wiff	Wiff

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Discovery and validation of serum glycoprotein biomarkers for high grade serous ovarian cancer.

Dutt Mriga M Hartel Gunter G Richards Renee S RS Shah Alok K AK Mohamed Ahmed A Apostolidou Sophia S Gentry-Maharaj Aleksandra A Hooper John D JD Perrin Lewis C LC Menon Usha U Hill Michelle M MM

Proteomics. Clinical applications 20230601 4

<h4>Purpose</h4>This study aimed to identify serum glycoprotein biomarkers for early detection of high-grade serous ovarian cancer (HGSOC), the most common and aggressive histotype of ovarian cancer.<h4>Experimental design</h4>The glycoproteomics pipeline lectin magnetic bead array (LeMBA)-mass spectrometry (MS) was used in age-matched case-control serum samples. Clinical samples collected at diagnosis were divided into discovery (n = 30) and validation (n = 98) sets. We also analysed a set of p ...[more]

PMID: 37147936

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