Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Escherichia Coli
SUBMITTER: John Young
LAB HEAD: Franck Duong
PROVIDER: PXD032315 | Pride | 2022-06-09
REPOSITORIES: Pride
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DY330_detergent_rep1.raw | Raw | |||
DY330_detergent_rep2.raw | Raw | |||
DY330_detergent_rep3.raw | Raw | |||
DY330_peptidisc_rep1.raw | Raw | |||
DY330_peptidisc_rep2.raw | Raw |
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Journal of proteome research 20220526 7
The peptidisc membrane mimetic enables global reconstitution of the bacterial membrane proteome into water-soluble detergent-free particles, termed peptidisc libraries. We present here a method that combines peptidisc libraries and chromosomal-level gene tagging technology with affinity purification and mass spectrometry (AP/MS) to stabilize and identify fragile membrane protein complexes that exist at native expression levels. This method circumvents common artifacts caused by bait protein over ...[more]