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Uterine extracellular vesicles in the mare.

ABSTRACT: The protein cargos of uterine extracellular vesicles isolated from uterine lavages, collected from pregnant mares (P; day 10, 11, 12 and 13 after insemination) and cyclic control mares (C; day 10 and 13 after insemination), was analyzed.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Equus Caballus (horse)

SUBMITTER: Thomas Fröhlich

LAB HEAD: Thomas Fröhlich

PROVIDER: PXD032405 | Pride | 2022-10-15

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	EVs_10_P13.raw	Raw
	EVs_11_Co10.raw	Raw
	EVs_12_Co13.raw	Raw
	EVs_13_Co10.raw	Raw
	EVs_14_Co13.raw	Raw

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Publications

Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare.

Rudolf Vegas Alba A Hamdi Meriem M Podico Giorgia G Bollwein Heinrich H Fröhlich Thomas T Canisso Igor F IF Bauersachs Stefan S Almiñana Carmen C

Scientific reports 20220916 1

In contrast to other domestic mammals, the embryo-derived signal(s) leading to maternal recognition of pregnancy (MRP) are still unknow in the mare. We hypothesize that these embryonic signals could be packed into uterine extracellular vesicles (uEVs), acting as multi-signal messengers between the conceptus and the maternal tract, and contributing to MRP. To unveil these signals, the RNA and protein cargos of uEVs isolated from uterine lavages collected from pregnant mares (P; day 10, 11, 12 and ...[more]

PMID: 36114358

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Isolation and characterization of equine uterine extracellular vesicles: a comparative methodological study

Project description:Extracellular vesicles (EVs) have been identified in the uterine fluid in different species and pointed as key players in the embryo-maternal dialogue, maternal recognition of pregnancy (MRP) and establishment of pregnancy. However, little is known about the uterine EVs in the mare. Therefore, the present study aimed at characterizing EVs from uterine lavage of cyclic mares by comparing five EVs isolation methods and the combination of them: (1) Ultracentrifugation (UC); (2) Concentration of lavage volume by Centricon ultrafiltration (CE); (3) the use of CE with different washing steps (PBS versus trehalose); (4) Size-exclusion chromatography with iZON-qEV columns to yield high-purity EVs and (5) a combination of the methods with best results based on EVs yield, purity, and protein cargo profiles. Transmission electron microscopy and Western blotting confirmed the isolation of EVs by all methods but with quantitative and qualitative differences. Mass spectrometry provided different protein profiles among methods with marked differences in the number of proteins and protein classes. Our results indicate that the combination of CE/trehalose/iZON/UC is an optimal method to isolate equine uterine EVs with good yield and purity that can be applied in future studies to determine the role of uterine EVs during MRP in the mare.

2021-09-09 | PXD022264 | Pride

Proteomic dataset of uterine fluid from mares diagnosed with post-breeding and infectious endometritis

Project description:This dataset contains raw and processed data from a proteomic analysis of uterine fluid from mares diagnosed with post-breeding and infectious endometritis, acquired via LC-MS/MS.

2024-10-16 | PXD056830 | JPOST Repository

Differential Gene Expression in Uterine Endometrium during Implantation in Pigs

Project description:This study provide an opportunity to elucidate the genetic control of fetal implantation and improve our understanding of fetal implantation and gestation maintenance, thus make further improvement for litter size of pigs. Nine pregnant sows were slaughtered by electrical on day 13, day 18 and day 24 after insemination (the pregnant group, three sows every period). The non-pregnant sows were slaughtered on day 13 after inseminated (n=3).In pregnant sows, samples of the endometrium attachment sites and inter-sites were taken. Samples from the endometrium of the non-pregnant sows were taken from comparable locations.

2015-01-26 | E-GEOD-51970 | biostudies-arrayexpress

Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation

Project description:Uterine glands are essential for pregnancy in mice and likely humans, because they secrete or transport bioactive substances that regulate uterine receptivity for blastocyst implantation. In mice, the uterus becomes receptive to blastocyst implantation on day 4, but is refractory by day 5. Here, blastocysts could be recovered from progesterone-induced uterine gland (PUGKO) but not wildtype (WT) mice on day 5 post-mating. Anti-adhesive Muc1 protein and microvilli were present on the luminal epithelium of PUGKO but not WT uteri. A number of known uterine receptivity genes and gland-specific genes were altered in the PUGKO uterus. Next, the uterus and uterine luminal fluid (ULF) were obtained from WT and PUGKO mice on day 3, 4 and 5. Transcriptome analysis revealed that 580 genes were decreased in the PUGKO uterus, however ULF secrotome analysis revealed that many proteins and several amino acids were increased in the PUGKO ULF. Of note, many proteins encoded by many gland-specific genes were not identified in the ULF of WT mice. These results support the ideas that uterine glands secrete factors that regulate ULF homeostasis and interact with other cell types in the uterus to influence uterine receptivity and blastocyst implantation for the establishment of pregnancy.

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Effect of dietary n-3 PUFA supplementation and post-insemination plane of nutrition on global gene expression patterns in bovine uterine endometrial tissue

Project description:The objectives of this study were to examine the effect of (i) dietary supplementation with n-3 PUFA and (ii) post-insemination plane of nutrition on the global transcriptome of uterine endometrial tissue, with particular focus on key genes and pathways that may affect pregnancy outcome. A total of 60 estrous-synchronised continental crossbred heifers were offered a concentrate-based diet supplemented (n-3 PUFA; n=32) or not (Control; n=28) with an n-3 PUFA-enriched product for 30 days prior to insemination. Following insemination, animals were allocated, within treatment, to one of two post insemination diets, either remaining on their existing high plane of nutrition (Control + High (n = 14); n-3 PUFA + High (n = 15)), or fed a low plane of nutrition diet (Control + Low (n = 14); n-3 PUFA + Low (n = 17)). Uterine endometrial tissue was collected, RNA isolated and gene expression analysis conducted using RNAseq. Comparison of transcript abundance across groups, highlighted a significant effect of diet on the endometrial transcriptome. The mTOR signalling pathway as well as pathways involved in early pregnancy/fertility was significantly enriched as a consequence of diet.

2020-12-07 | GSE141937 | GEO

Maternal recognition of pregnancy in the horse: are microRNAs the secret messengers? [miRNA-seq]

Project description:The signal for maternal recognition of pregnancy (MRP) has still not been identified in the horse. Endometrial biopsies, uterine fluid, embryonic tissues and yolk sac fluid were collected 13 days after ovulation during pregnant and control cycles from the same mares. Micro-RNA-Sequencing was performed on all collected samples and mRNA-Sequencing was conducted on the same endometrial and embryonic tissue samples

2020-02-24 | GSE141443 | GEO

Maternal recognition of pregnancy in the horse: are microRNAs the secret messengers? [RNA-seq]

2020-02-24 | GSE141360 | GEO

Bovine uterine fluid extracellular vesicle proteome on different timepoints of the oestrous cycle

Project description:Healthy cow uterine fluid (UF) was collected from synchronized cattle at day 0, 7 and 16 after ovulation. Extracellular vesicles (EVs) were isolated from UF. UF-EVs from same cattle on different timepoints were subjected to LC-MS/MS. Additionally, one sample before EV purification was subjected to LC-MS/MS (corresponding EV sample is named 1D0).

2024-01-26 | PXD040201 | Pride

Proteome of pig uterine luminal fluid

Project description:Proteome of pig uterine luminal fluid on day 9 of estrus and days 9, 12 and 15 of pregnancy

2021-08-06 | PXD026504 |

Gene expression profiling in periimplantation mouse uterine luminal epithelium

Project description:We used the microarray analysis to determine the differential gene expression profiles in mouse uterine luminal epithelium between preimplantation gestation day 3.5 and postimplantation gestation day 4.5, and investigeate the molecular mechanism of the establishment of uterine receptivity and embryo implantation. Uterine luminal epithelium (LE) is critical for the establishment of uterine receptivity during embryo implantation. Many genes are known to have differential expression in the periimplantation LE but the global profiling of the altered genes in the periimplantation LE is unknown. To fill in this knowledge gap, microarray analysis was performed in gestation day 3.5 (D3.5, preimplantation) and D4.5 (postimplantation) mouse LE from natural pregnancy. There were 382 significantly upregulated and 245 significantly downregulated genes (>2 fold, P<0.05) in the D4.5 LE.

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