Dataset Information

Quantitative Proteomics Analysis to Assess Protein Expression Levels in the Ovaries of Pubescent Goats

ABSTRACT: Herein we identified 301 (176 up- and 125 downregulated) differentially abundant proteins (DAPs) in the ovaries of pubertal and prepubertal Anhui white goats (P < 0.05) using quantitative proteomics techniques based on LC–MS/MS and iTRAQ. Gene ontology functional enrichment analysis revealed that several DAPs enriched in biological processes were related to cellular process, biological regulation, metabolic process, and response to stimulus. Further, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that DAPs were enriched in olfactory translation, glutathione metabolism, and calcium signaling pathways.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Capra Hircus (goat)

TISSUE(S): Ovary

SUBMITTER: Ping Qin

LAB HEAD: Fang Fugui

PROVIDER: PXD032665 | Pride | 2022-08-12

REPOSITORIES: pride

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			Action	DRS
	20P1306_all.mgf	Mgf
	Exploris_XM_HD_20210103_20P1306_1.raw	Raw
	Exploris_XM_HD_20210103_20P1306_10.raw	Raw
	Exploris_XM_HD_20210103_20P1306_11.raw	Raw
	Exploris_XM_HD_20210103_20P1306_12.raw	Raw

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Quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats.

Qin Ping P Ye Jing J Gong Xinbao X Yan Xu X Lin Maosen M Lin Tao T Liu Tong T Li Hailing H Wang Xiujuan X Zhu Yanyun Y Li Xiaoqian X Liu Ya Y Li Yunsheng Y Ling Yinghui Y Zhang Xiaorong X Fang Fugui F

BMC genomics 20220713 1

<h4>Background</h4>Changes in the abundance of ovarian proteins play a key role in the regulation of reproduction. However, to date, no studies have investigated such changes in pubescent goats. Herein we applied isobaric tags for relative and absolute quantitation (iTRAQ) and liquid chromatography-tandem mass spectrometry to analyze the expression levels of ovarian proteins in pre-pubertal (n = 3) and pubertal (n = 3) goats.<h4>Results</h4>Overall, 7,550 proteins were recognized; 301 (176 up- a ...[more]

PMID: 35831802

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