Dataset Information

Proteomic Analysis of dental pulp stem cell-derived exosomes

ABSTRACT: In order to examine the proteomics of exosomes derived from DPSCs under hypoxia (Hypo-Exos) and the mechanism underlying the angiogenic effect of DPSC-Exos, iTRAQ-based proteomics analysis and bioinformatic analysis were performed to investigate proteome profile differences between exosomes derived from DPSCs under normoxia (Nor-Exos) and Hypo-Exos.7114 peptides and 1792 proteins were identified from the Hypo-Exos samples with a 1% FDR. Compared with the control group, 39 proteins were upregulated and 40 proteins were downregulated in Hypo-Exos.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Dental Pulp

SUBMITTER: Xuehong Xian

LAB HEAD: Qimei Gong

PROVIDER: PXD032719 | Pride | 2022-05-20

REPOSITORIES: Pride

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Hypoxia Alters the Proteome Profile and Enhances the Angiogenic Potential of Dental Pulp Stem Cell-Derived Exosomes.

Li Baoyu B Xian Xuehong X Lin Xinwei X Huang Luo L Liang Ailin A Jiang Hongwei H Gong Qimei Q

Biomolecules 20220414 4

Dental pulp stem cells (DPSCs) and their exosomes (Exos) are effective treatments for regenerative medicine. Hypoxia was confirmed to improve the angiogenic potential of stem cells. However, the angiogenic effect and mechanism of hypoxia-preconditioned DPSC-Exos are poorly understood. We isolated exosomes from DPSCs under normoxia (Nor-Exos) and hypoxia (Hypo-Exos) and added them to human umbilical vein endothelial cells (HUVECs). HUVEC proliferation, migration and angiogenic capacity were asses ...[more]

PMID: 35454164

Similar Datasets

Project description:Rationale：Poor peri-implant osseointegration of dental implants in patients with type II diabetes has become a major clinical challenge in recent years. MSC (Mesenchymal stem cell)-derived exosomes may play an important role in peri-implant osseointegration, but the mechanism remains unclear. Enhancing the therapeutic effect of MSC-derived exosomes and exploring the potential mechanism can help provide a new therapeutic strategy to improve the clinical outcome of dental implant restorations in patients with type II diabetes. Methods：The exosomes derived from hypoxia (Hypo-exos) or normoxia (Nor-exos) preconditioned bone marrow mesenchymal stem cells (BMSCs) were co-cultured with BMSCs and human umbilical vein endothelial cells (HUVECs). The effect of exosomes on BMSCs cell proliferation was detected by CCK-8 assay and EdU assay, and the effect on angiogenesis ability of HUVECs was detected by wound healing assay, transwell migration assay, tube formation assay, enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. A diabetic rat dental implant model was also established and the effect of exosomes on implant osseointegration was evaluated through micro-CT scanning and histological analysis. The differentially expressed miRNAs between Hypo-exos and Nor-exos were identified by high-throughput miRNA sequencing. Subsequently, the target genes and their roles in regulating angiogenesis were predicted and analyzed by bioinformatics analysis and dual luciferase reporter assay. Results：In vitro experiments indicated that hypoxia preconditioning could elevate exosome production and promote cell proliferation of BMSCs and angiogenesis of HUVECs. Moreover, Hypo-exos promoted peri-implant osteogenesis in rats with diabetes. Further investigation revealed the vital involvement of the miR-106b-5p/HIF-1α axis in promoting peri-implant osseointegration. Conclusion: Exosomes derived from hypoxia-preconditioned BMSCs could improve the peri-implant osseointegration in rats with diabetes by promoting cell proliferation and angiogenesis, and the miR-106b-5p/ HIF-1α axis could be the underlying mechanism.

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	P170435_1.mgf	Mgf
	P170435_1.wiff	Wiff
	P170435_1.wiff.scan	Wiff
	P170435_10.mgf	Mgf
	P170435_10.wiff	Wiff