Proteomics

Dataset Information

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Development of functional anti-Gn nanobodies specific for SFTSV based on next generation sequencing and proteomics


ABSTRACT: Severe fever with thrombocytopenia syndrome (SFTS) is an acute infectious disease caused by novel bunyavirus (SFTSV), with a mortality rate of 6.3% ~ 30%. To date, there is no specific treatment for SFTS. Previously, our studies demonstrate that SFTSV surface glycoprotein (Glycoprotein N, Gn) is a potential target for the development of SFTS vaccine or therapeutic antibodies, and anti-Gn neutralizing antibodies play a protective role in SFTS infection. Compared with traditional antibodies, nanobodies from camelids have various advantages including small molecular weight, high affinity, low immunogenicity and convenient production by gene engineering, etc. In this study, we combined next generation sequencing (NGS) with proteomics technology and bioinformatics analysis to high-throughput screen monoclonal anti-Gn nanobodies from camel immunized with Gn protein. We identified 19 anti-Gn monoclonal nanobody sequences, and selected 6 of them for recombinant protein expression and purification. Among these 6 anti-Gn nanobodies, nanobody 57493 was validated to be highly specific for Gn.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Camelidae

TISSUE(S): Blood Serum

SUBMITTER: Lei Fang  

LAB HEAD: Lei Fang

PROVIDER: PXD032731 | Pride | 2024-01-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20190424_07.wiff Wiff
20190424_07.wiff.scan Wiff
20190424_07_GN_AspN_PeptideSummary.txt Txt
20190424_07_GN_AspN_ProteinSummary.txt Txt
20190424_08.wiff Wiff
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Publications

Development of functional anti-Gn nanobodies specific for SFTSV based on next-generation sequencing and proteomics.

Wang Binghao B   Huang Bilian B   Li Xinyu X   Guo Yan Y   Qi Guantong G   Ding Yibing Y   Gao Haidong H   Zhang Jingzi J   Wu Xilin X   Fang Lei L  

Protein science : a publication of the Protein Society 20221101 11


Severe fever with thrombocytopenia syndrome (SFTS) is an acute infectious disease caused by novel bunyavirus (SFTSV), with a mortality rate of 6.3% ~ 30%. To date, there is no specific treatment for SFTS. Previously, we demonstrated that SFTSV surface glycoprotein (Glycoprotein N, Gn) was a potential target for the development of SFTS vaccine or therapeutic antibodies, and anti-Gn neutralizing antibodies played a protective role in SFTS infection. Compared with traditional antibodies, nanobodies  ...[more]

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