Dataset Information

Short and mid term heat stress proteomics characterization in pinus radiata

ABSTRACT: In order to study in detail short and mid term heat stress molecular response we performed a shotgun proteomics study. One-year-old P. radiata seedlings (plant size ∼33 ± 4 cm) were kept in 1 dm3 pots under a photoperiod of 16 h (400 µmol m−2 s−1) at 25 °C and 50% relative humidity (RH), and 15 °C and 60% RH during the night period. The plants were previously acclimated over a 1-month period inside the climate chamber, being watered with nutritive solution (N : P : K, 5 : 8 : 10). In order to perform a realistic experiment, and corresponding with the dawn, heat exposure treatment began with an increasing temperature gradient from 15 to 40 °C over 5 h, which was then maintained for 6 h; after that, temperature was gradually decreased from 40 to 15 °C over 5 h. This experimental procedure was repeated for 5 days and sampling was performed at: 3 h after 40 °C was reached on Day 1 (T1/2) and at the end of the 6 h heat exposure on Day 1 (T1), Day 2 (T2), Day 3 (T3) and Day 5 (T5) (Figure 1a). Plants were watered every day to 80% full capacity (FC) in order to avoid a collateral drought stress. A group of control plants was also collected on Day 1 (C) before starting the heat exposure.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Pinus Radiata (monterey Pine)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Laura Lamelas

LAB HEAD: Mónica Meijón

PROVIDER: PXD032754 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MonicaMeijon_sol23_15_C_II_22072015.msf	Msf
	MonicaMeijon_sol23_15_C_II_22072015.raw	Raw
	MonicaMeijon_sol23_15_C_I_22072015.msf	Msf
	MonicaMeijon_sol23_15_C_I_22072015.raw	Raw
	MonicaMeijon_sol23_15_C_V_22072015_150914161917.msf	Msf

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Publications

Integrative analysis in Pinus revealed long-term heat stress splicing memory.

Roces Víctor V Lamelas Laura L Valledor Luis L Carbó María M Cañal María Jesús MJ Meijón Mónica M

The Plant journal : for cell and molecular biology 20221013 4

Due to the current climate change, many studies have described main drivers in abiotic stress. Recent findings suggest that alternative splicing (AS) has a critical role in controlling plant responses to high temperature. AS is a mechanism that allows organisms to create an assortment of RNA transcripts and proteins using a single gene. However, the most important roles of AS in stress could not be rigorously addressed because research has been focused on model species, covering only a narrow ph ...[more]

PMID: 36151923

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