Proteomics

Dataset Information

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SWATH-MS based characterization for proteome profile data of chemicals


ABSTRACT: Profile data analysis is useful to reveal the mode of action of low molecular weight compounds. Although transcriptome data have been the main target of the profile data analysis, it is unknown whether omics data in different layers could deserve the profile data analysis. In the first place, what is not described as numerical data does not serve for analyses. Therefore, understanding the characteristics of omics data in different layers is crucial. In this study, we examined whether proteome data obtained by SWATH-MS (Sequential Window Acquisition of all Theoretical Mass Spectra) is useful to understand the mode of action of low molecular weight compounds. We demonstrated that proteome data obtained by SWATH-MS was useful for profile data analyses to the same extent as transcriptome data. Furthermore, we revealed a new mode of action of natural compound harmine as a result of profile data analysis using SWATH-MS data.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Risa Sato  

LAB HEAD: Yasuo Uchida

PROVIDER: PXD032785 | Pride | 2023-02-22

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
190724_OLSA_IDA_DMSO_6.wiff Wiff
190724_OLSA_IDA_DMSO_6.wiff.scan Wiff
190724_OLSA_SWATH_4-PBA_1.wiff Wiff
190724_OLSA_SWATH_4-PBA_1.wiff.scan Wiff
190724_OLSA_SWATH_4-PBA_2.wiff Wiff
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