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Barley newly synthesized ribosomal proteome: E.coli control samples

ABSTRACT: Ribosomal proteome profiling control for project: Plants synthesize new ribosomal proteins and recycle others in cold-acclimated ribosomes and these in turn synthesize a differential proteome in barley root meristems.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Escherichia Coli

SUBMITTER: Federico Martinez-Seidel

LAB HEAD: Berin A. Boughton

PROVIDER: PXD032938 | Pride | 2024-05-23

REPOSITORIES: Pride

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Publications

Streamlining Protein Fractional Synthesis Rates Using SP3 Beads and Stable Isotope Mass Spectrometry: A Case Study on the Plant Ribosome.

Gentry-Torfer Dione D Murillo Ester E Barrington Chloe L CL Nie Shuai S Leeming Michael G MG Suwanchaikasem Pipob P Williamson Nicholas A NA Roessner Ute U Boughton Berin A BA Kopka Joachim J Martinez-Seidel Federico F

Bio-protocol 20240505 9

Ribosomes are an archetypal ribonucleoprotein assembly. Due to ribosomal evolution and function, r-proteins share specific physicochemical similarities, making the riboproteome particularly suited for tailored proteome profiling methods. Moreover, the structural proteome of ribonucleoprotein assemblies reflects context-dependent functional features. Thus, characterizing the state of riboproteomes provides insights to uncover the context-dependent functionality of r-protein rearrangements, as the ...[more]

PMID: 38737506

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