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PRODIGE, Digestomic data of proteolysis after tanin supplementation in the rumen

ABSTRACT: The main objective of this study was to investigate the effect of tannins on the fate of dietary protein throughout the ruminant digestive tract using a dynamic in vitro digester under controlled conditions

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive HF

ORGANISM(S): Medicago Sativa Subsp. X Varia

TISSUE(S): Rumen, Gastric Juice

SUBMITTER: christophe Chambon

LAB HEAD: Christophe Chambon

PROVIDER: PXD032973 | Pride | 2022-06-14

REPOSITORIES: Pride

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			Action	DRS
	7740_P08_G60_T_.raw	Raw
	7741_P05_G60_ST_.raw	Raw
	7742_P03_G15_ST_.raw	Raw
	7743_P04_G15_T_.raw	Raw
	7744_P03_G60_ST_.raw	Raw

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Publications

Impact of Tannin Supplementation on Proteolysis during Post-Ruminal Digestion in Wethers Using a Dynamic In Vitro System: A Plant (Medicago sativa) Digestomic Approach.

Sayd Thierry T Chambon Christophe C Popova Milka M Morgavi Diego P DP Torrent Angélique A Blinet Sylvie S Theron Laetitia L Niderkorn Vincent V

Journal of agricultural and food chemistry 20220214 7

The aim of this study was to characterize the effects of tannins on plant protein during sheep digestion using a digestomic approach combining in vivo (rumen) conditions and an in vitro digestive system (abomasum and small intestine). Ruminal fluid from wethers infused with a tannin solution or water (control) was introduced into the digester, and protein degradation was followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Tannin infusion in the rumen led to a cle ...[more]

PMID: 35157803

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Digestomic data of proteolysis during whether post rumen digestion after tannin supplementation.

Project description:The protein degradation of alfalfa hay after tannin supplementation was monitored during wethers digestion. Three rumen-cannulated wethers were infused a tannin solution, and water for control, through the cannula. The digestion time-points samples were collected in vivo in the rumen and in vitro in the abomasum, and the small intestine compartments. The digestomic dataset was acquired by identifying and quantifying the peptides resulting from the protein degradation, using high-resolution LC-MS/MS mass spectrometry and label-free quantitation. The digestomic dataset is the compilation of proteomic data acquired in the rumen and peptidomic data acquired in the abomasum and in the small intestine. The proteomic analysis identified 20 Medicago proteins in the rumen fluid, based on 169 peptides of which 140 are unique. The peptidomic analysis identified 28 Medicago proteins in the abomasum, based on 575 peptides of which 363 are unique, and 11 Medicago proteins in the small intestine, based on 94 peptides of which 63 are unique. This digestomic dataset of proteolysis during sheep post rumen digestion after tannin supplementation reveals the protein regions protected by tannin supplementation, and could be reused in studies related to the protein use efficiency by ruminants.

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