Dataset Information

LIS1 RNA binding orchestartes the mechanosensitive properties of embryonic stem cells in AGO2 dependent and independent ways.

ABSTRACT: LIS1 immunoprecipitation in nuclear and cytoplasmic fractions of LIS1 Floxed/-, OE and WT embryonic stem cells. To identify AGO2 dependent and independent interactions LIS1 was immunoprecipitated in AGO1-4 knockout and doxycycline induced AGO2 mouse embryonic stem cells.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Embryonic Stem Cell

DISEASE(S): Lissencephaly 1

SUBMITTER: Aditya Kshirsagar

LAB HEAD: Orly Reiner

PROVIDER: PXD033150 | Pride | 2023-07-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	160208_Sample-01_Cyto-WT-BeadsOnly_300nL.raw	Raw
	160208_Sample-02_Cyto-Het-BeadsOnly_300nL.raw	Raw
	160208_Sample-03_Cyto-OE-BeadsOnly_300nL.raw	Raw
	160208_Sample-04_Cyto-WT-LIS1_300nL.raw	Raw
	160208_Sample-05_Cyto-Het-LIS1_300nL.raw	Raw

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Publications

LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways.

Kshirsagar Aditya A Doroshev Svetlana Maslov SM Gorelik Anna A Olender Tsviya T Sapir Tamar T Tsuboi Daisuke D Rosenhek-Goldian Irit I Malitsky Sergey S Itkin Maxim M Argoetti Amir A Mandel-Gutfreund Yael Y Cohen Sidney R SR Hanna Jacob H JH Ulitsky Igor I Kaibuchi Kozo K Reiner Orly O

Nature communications 20230606 1

Lissencephaly-1 (LIS1) is associated with neurodevelopmental diseases and is known to regulate the molecular motor cytoplasmic dynein activity. Here we show that LIS1 is essential for the viability of mouse embryonic stem cells (mESCs), and it governs the physical properties of these cells. LIS1 dosage substantially affects gene expression, and we uncovered an unexpected interaction of LIS1 with RNA and RNA-binding proteins, most prominently the Argonaute complex. We demonstrate that LIS1 overex ...[more]

PMID: 37280197

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