Proteomics

Dataset Information

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A mechanism of gene evolution generating mucin function


ABSTRACT: How novel gene functions evolve is a fundamental question in biology. Mucin proteins, a functionally but not evolutionarily defined group of proteins, allow to study convergent evolution of gene function. By analyzing the genomic variation of mucins across a wide range of mammalian genomes, we propose that exonic repeats and their copy number variation contribute substantially to the de novo evolution of new gene functions. By integrating bioinformatic, phylogenetic, proteomic, and immunohistochemical approaches, we identified 15 undescribed instances of evolutionary convergence, where novel mucins originated by gaining densely O-glycosylated exonic repeat domains. Our results suggest that secreted proteins rich in proline are natural precursors for acquiring mucin function. Our findings have broad implications for understanding the role of exonic repeats in the parallel evolution of new gene functions, especially those involving protein glycosylation.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Rattus Norvegicus (rat) Bos Taurus (bovine) Homo Sapiens (human) Mustela Putorius Furo (european Domestic Ferret) (mustela Furo) Sus Scrofa Domesticus (domestic Pig) Mus Musculus (mouse) Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Saliva

SUBMITTER: Shichen Shen  

LAB HEAD: Jun Qu

PROVIDER: PXD033197 | Pride | 2022-10-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Gel_Cow_C1_120K_HCD_IT_150min.msf Msf
Gel_Cow_C1_120K_HCD_IT_150min.raw Raw
Gel_Cow_C2_120K_HCD_IT_150min.msf Msf
Gel_Cow_C2_120K_HCD_IT_150min.raw Raw
Gel_Dog_D1_120K_HCD_IT_150min.msf Msf
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Publications

A mechanism of gene evolution generating mucin function.

Pajic Petar P   Shen Shichen S   Qu Jun J   May Alison J AJ   Knox Sarah S   Ruhl Stefan S   Gokcumen Omer O  

Science advances 20220826 34


How novel gene functions evolve is a fundamental question in biology. Mucin proteins, a functionally but not evolutionarily defined group of proteins, allow the study of convergent evolution of gene function. By analyzing the genomic variation of mucins across a wide range of mammalian genomes, we propose that exonic repeats and their copy number variation contribute substantially to the de novo evolution of new gene functions. By integrating bioinformatic, phylogenetic, proteomic, and immunohis  ...[more]

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