Proteomics

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Label free quantitative proteomic analysis on (-)-epicatechin (EC) and 5-(3',4'-dihydroxyphenyl)-γ-valerolactone (3,4-diHPV) treated Caco-2 and Hepa1c1c7 cells

ABSTRACT: Epicatechin (EC) is a well-known dietary polyphenol and widely found in various foods, e.g., apple, cocoa, green tea, wine, etc. Its consumption has been associated with attenuation of some chronic diseases. After ingestion of EC, a large portion is passed into the large intestinal lumen where it is extensively degraded into various microbial metabolites. One of the major EC microbial metabolites is 5-(3’,4’-dihydroxyphenyl)-γ-valerolactone (3,4-diHPV). The protein expression profiles of EC or 3,4-diHPV treated Caco-2 and Hepa1c1c7 cells were studied with the help of label-free quantitative proteomics. Compared to the parent compound EC, 3,4-diHPV was able to induce upregulations of more Nrf2-related proteins in both cell lines, and the number of significant differentially expressed proteins (DEPs) induced by 3,4-diHPV in Hepa1c1c7 cells was much higher than the respective EC treatment.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Sjef Boeren  

LAB HEAD: Sjef Boeren

PROVIDER: PXD033248 | Pride | 2022-08-12

REPOSITORIES: Pride

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Dataset's files

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Action DRS
CLi44_Control_rep1.raw Raw
CLi45_Exp_EGCG_rep1.raw Raw
CLi46_Exp_EGC_rep1.raw Raw
CLi47_Exp_EC_rep1.raw Raw
CLi48_Exp_PV_rep1.raw Raw
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Publications

Intra- and Inter-individual Differences in the Human Intestinal Microbial Conversion of (-)-Epicatechin and Bioactivity of Its Major Colonic Metabolite 5-(3',4'-Dihydroxy-Phenyl)-γ-Valerolactone in Regulating Nrf2-Mediated Gene Expression.

Liu Chen C   Boeren Sjef S   Rietjens Ivonne M C M IMCM  

Frontiers in nutrition 20220630

(-)-Epicatechin (EC) is one of the most popular polyphenols present in various food products in daily life. Upon intake, it is intensively metabolized by microbiota in the large intestine. In the present study, intra- and inter-individual variations in this gut microbial conversion of EC and the concomitant formation of its major metabolites, including 5-(3',4'-dihydroxy phenyl)-γ-valerolactone (3,4-diHPV), were identified and quantified <i>via</i> liquid chromatography triple quadrupole mass sp  ...[more]

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