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Silent recognition by Toll-like receptor 5 of flagellins from human gut commensal bacteria

ABSTRACT: Flagellins from commensal bacteria can be weak Toll-like receptor (TLR)5 agonists despite high affinity binding to TLR5, ligands we termed “silent flagellins”. To determine if silent flagellins are detectable in the human gut, endogenous flagellins produced by the microbiota were isolated from stool obtained from a healthy adult female donor. TLR5 was used as bait to enrich for silent flagellins and TLR5-bound flagellins were identified by searching peptides against a custom flagellin database built from metagenome sequences.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Irina Droste-Borel

LAB HEAD: Ruth E. Ley

PROVIDER: PXD033249 | Pride | 2022-10-12

REPOSITORIES: Pride

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			Action	DRS
	20210705_CO_1033RuLe_R01.raw	Raw
	Flagellin_Top_Abund_10.fasta	Fasta
	TLR5-HA.fasta	Fasta
	UP000005640_9606_complete_2020-10-07.fasta	Fasta
	checksum.txt	Txt

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