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UHMK1 is a novel splicing regulatory kinase

ABSTRACT: The U2AF Homology Motif Kinase 1 (UHMK1) is the only kinase that contains the U2AF homology motif (UHM), a common protein interaction domain among splicing factors. Through this motif, UHMK1 interacts with the splicing factors SF1 and SF3B1, known to participate in the 3’ splice site recognition and early steps of spliceosome assembly. Also, UHMK1 phosphorylates these splicing factors in vitro. However, the involvement of UHMK1 in RNA processing has not previously been demonstrated. Here, we used global phosphoproteomics to identify novel putative substrates in UHMK1-overexpressing or -depleted (knockdown) NIH3T3 cells and better characterize the function of this kinase in splicing and other biological processes.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Fibroblast

SUBMITTER: Vanessa Arfelli

LAB HEAD: Leticia Fröhlich Archangelo

PROVIDER: PXD033353 | Pride | 2023-05-02

REPOSITORIES: Pride

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			Action	DRS
	03489_BA1_P040688_S00_I00_U01_R1_TMT10.raw	Raw
	03489_BA2_P040689_S00_I00_U01_R1_TMT10.raw	Raw
	03489_BA3_P040690_S00_I00_U01_R1_TMT10.raw	Raw
	03489_BA4_P040685_S00_A00_R1_TMT10.raw	Raw
	03489_BB1_P040688_S00_I00_U02_R1_TMT10.raw	Raw

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