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Analysis of differentially abundant proteins in sugarcane during red rot infection

ABSTRACT: The project is aimed at analysing the comparitive proteomics of red rot resistant cultivar (BO91) and red rot susceptible cultivar (COJ64) of sugarcane. The differentially abundant proteins shall be used to identify the corresponding genes.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Saccharum Hybrid Cultivar Bo91 Saccharum Hybrid Cultivar Coj64

TISSUE(S): Stalk Cell

SUBMITTER: Ranjit Singh Gujjar

LAB HEAD: Ranjit Singh Gujjar

PROVIDER: PXD033375 | Pride | 2023-03-23

REPOSITORIES: Pride

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Project description:Noble rot results from atypical infections of ripe grape berries by Botrytis cinerea. Unlike bunch rot, noble rot promotes favorable changes in grape berries and accumulation of secondary metabolites that enhance wine grape quality. Noble rot-infected berries of SÃ©millon, a white-skinned variety, were collected over three years from a commercial vineyard at the same time fruit were harvested for botrytized wine production. Transcriptomic and metabolomic data were integrated to identify pathways associated with distinct stages of noble rot. Botrytis induced the expression of known key regulators of pathways in secondary metabolism associated with berry ripening. The activation by Botrytis during noble rot of metabolic pathways associated with berry ripening was further supported by comparisons with transcriptomes of red-skinned varieties at vÃ©raison. A prominent and common outcome of noble rot and berry ripening was the enhancement of the phenylpropanoid metabolism. Induced synthesis of stilbenes, flavonoids, and anthocyanins was supported by both transcriptional and metabolite analyses. Enzyme assays and targeted gene expression analyses of samples from the three distinct years confirmed that the activation of central and peripheral phenylpropanoid pathways is a consistent hallmark of noble rot. Finally, we show that the impact of noble rot on grape metabolism is still detectable in botrytized wines. These results demonstrate that despite the late stage of terminal senescence of a plant organ, a biotic stress can cause a major reprogramming of plant metabolism leading, in case of noble rot, to the synthesis of important metabolites for grape berry flavor and aroma. The experimental design consists of 4 treatments, berries at 3 distinct stages of noble rot and berries without apparent symptoms of infection. From each treatment a total of 4 biological replicates were sequenced with RNAseq, each replicate corresponded to independent pools of 10-15 berries harvested from adjacent grapevines.

			Action	DRS
	Sugarcane_8SAMPLES_Raw_data.rar	Other
	_CHRO001.DAT	Other
	_CHRO002.DAT	Other
	_CHROMS.INF	Other
	_FUNC001.DAT	Other

Dataset Information

Analysis of differentially abundant proteins in sugarcane during red rot infection

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