Proteomics

Dataset Information

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A Bioorthogonal Chemical Reporter for the Detection and Identification of Protein Lactylation


ABSTRACT: L-Lactylation is a recently discovered post-translational modification occurring on histone lysine residues to regulate gene expression. However, the substrate scope of lactylation, especially that in non-histone proteins, remains unknown, largely due to the limitations of current methods for analyzing lactylated proteins. Herein, we report an alkynyl-functionalized bioorthogonal chemical reporter, YnLac, for the detection and identification of protein lactylation in mammalian cells. Our in-gel fluorescence and chemical proteomic analyses show that YnLac is metabolically incorporated into lactylated proteins and directly labels known lactylated lysines of histones. We further apply YnLac to the proteome-wide profiling of lactylation, revealing many novel modification sites in non-histone proteins for the first time.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Tao Peng  

LAB HEAD: Tao Peng

PROVIDER: PXD033454 | Pride | 2022-06-23

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
YnLac_NonNuc_Cytoplasmic_fraction.zip Other
YnLac_NonNuc_Cytoplasmic_fraction_Search.zip Other
YnLac_Nuclear_fraction.zip Other
YnLac_Nuclear_fraction_Search.zip Other
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Publications

A bioorthogonal chemical reporter for the detection and identification of protein lactylation.

Sun Yanan Y   Chen Yanchi Y   Peng Tao T  

Chemical science 20220426 20


l-Lactylation is a recently discovered post-translational modification occurring on histone lysine residues to regulate gene expression. However, the substrate scope of lactylation, especially that in non-histone proteins, remains unknown, largely due to the limitations of current methods for analyzing lactylated proteins. Herein, we report an alkynyl-functionalized bioorthogonal chemical reporter, YnLac, for the detection and identification of protein lactylation in mammalian cells. Our in-gel  ...[more]

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