Proteomics

Dataset Information

0

Chiton shell proteome sheds light on stem-group mollusca with biomineralization toolkits


ABSTRACT: The appearance of hard mineralized exoskeletons is a critical leap for animal evolution and partially lead to the explosion of diverse animals during the Cambrian, for example, molluscs. A majority of molluscs have mineralized shells to protect themselves. Despite numerous studies that have studied the remarkable mechanical properties of shells, the origin of shell formation is still elusive. Hence, this study investigated the overlooked shell proteome of chitons, which belong to polyplacophoran, Aculifera of Mollusca. By comparing the shell proteome to well-studied Conchifera groups, we inferred possible ancestral biomineralization toolkits of stem-group Mollusca. Taking advantage of the recently sequenced chiton mantle transcriptome and genome, eight core biomineralization proteins were identified by proteomics. Surprisingly, in contrast to previous thought that shell formation is convergently evolved, two important shell matrix proteins, Nacrein-like and Pif-like proteins were found to be conserved among Aculifera and Conchifera groups. Our findings identify a missed link of mineralized shell evolution in Mollusca and pose a hypothesis that stem-group molluscs have already evolved core biomineralization toolkits, which likely facilitate the formation of mineralized shells for protection that partially leads to their explosion.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Chiton Stokesii

SUBMITTER: Chuang Liu  

LAB HEAD: Chuang Liu

PROVIDER: PXD033490 | Pride | 2023-01-03

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MS210653-1-chiton1.xlsx Xlsx
MS210653-1.raw Raw
MS210653-2-chiton1.xlsx Xlsx
MS210653-2.raw Raw
MS210653-3-chiton1.xlsx Xlsx
Items per page:
1 - 5 of 8

Similar Datasets

2010-02-05 | GSE14305 | GEO
2010-02-04 | E-GEOD-14305 | biostudies-arrayexpress
2017-07-07 | PXD006786 | Pride
2019-03-26 | PXD010130 | Pride
2010-02-05 | GSE13980 | GEO
2010-02-05 | GSE14303 | GEO
2022-01-09 | MSV000088664 | MassIVE
2019-03-20 | PXD013153 | JPOST Repository
2022-07-01 | PXD030901 | Pride
2010-05-14 | E-GEOD-13980 | biostudies-arrayexpress