Dataset Information

Surface proteome of emetic Bacillus cereus delta-sl2 delta-eag double mutant strain grown in glucose containing medium

ABSTRACT: his project provides tandem mass spectrometry data of extracellular non-covalently bound cell surface proteins from samples of Bacillus cereus AH187 devoid of the S-layer 2 (SL2) and extractable antigen 1 (EA1) S-layer proteins. The double mutant strain was grown on liquid MOD medium supplemented with glucose and harvested at five growth stages: early exponential, late exponential, stationary phase, tardive stationary 1 and tardive stationary 2.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bacillus Cereus Ah187

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD033494 | Pride | 2022-08-12

REPOSITORIES: Pride

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Publications

Dynamic Profile of S-Layer Proteins Controls Surface Properties of Emetic Bacillus cereus AH187 Strain.

Boutonnet Cécile C Lyonnais Sébastien S Alpha-Bazin Beatrice B Armengaud Jean J Château Alice A Duport Catherine C

Frontiers in microbiology 20220629

Many prokaryotes are covered by a two-dimensional array of proteinaceous subunits. This surface layers (S-layer) is incompletely characterized for many microorganisms. Here, we studied Bacillus cereus AH187. A genome analysis identified two genes encoding the S-layer proteins SL2 and EA1, which we experimentally confirmed to encode the two protein components of the S-layer covering the surface of B. cereus. Shotgun proteomics analysis indicated that SL2 is the major component of th ...[more]

PMID: 35847057

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Project description:A comparative transcriptome approach was used to assess genes involved in metabolism and pathogenesis that are specifically activated during anaerobic growth of the spore-forming food-borne human pathogen Bacillus cereus ATCC 14579. Growth under anaerobic conditions in Brain Heart Infusion broth revealed a reduced growth rate and a lower yield as compared to that under aerobic conditions. Comparative transcriptome analysis of cells harvested at early- and mid-exponential growth phase, transition phase and stationary phase, subsequently showed hundreds of genes to be induced under anaerobic condition. These included novel genes identified for anaerobic growth of B. cereus, encoding metabolic pathways, such as the arginine deiminase pathway (ArcABDC), a formate dehydrogenase (FdhF) and a pyruvate fomate lyase (Pfl), and alternative respiratory proteins, such as arsenate reductases. Furthermore, the nitrosative stress response was induced in the anaerobic transition phase of growth, conceivably due to the production of nitric oxide as a by-product of nitrite and nitrate respiration. Notably, both hemolytic enzyme and enterotoxin encoding genes were activated in different oxygen limiting conditions, i.e. hemolytic enzyme encoding genes were induced during anaerobic growth, whereas enterotoxin encoding genes were induced in the transition and stationary phase of aerobic cultures reaching a high cell density. These data point to metabolic rearrangements, stress adaptation and activation of the virulent status of B. cereus under anaerobic conditions, such as encountered in the human GI-tract. B. cereus ATCC 14579 was grown in BHI in 50 ml. Aerobic in a Erlenmeyer flask, shaking at 200 rpm. Anaerobic in a closed flask, flushed with Nitrogen-gas for 30 min, also shaking at 200 rpm. Transcriptome analyses Phase compared to mid-exponential phase Anaerobic (OD600) 0.2 compared to 0.4 Early-exponential 1.0 compared to 0.4 Transition 1.1 compared to 0.4 Stationary Aerobic (OD600) 0.2 compared to 0.8 Early-exponential 4.0 compared to 0.8 Transition 8.0 compared to 0.8 Stationary Aerobic to anaerobic (OD600) Anaerobic 0.6 to aerobic 0.6

			Action	DRS
	BcereusAH187_2020-06-22.fasta	Fasta
	F221731.dat	Other
	F221731_p0.05.mzid.gz	Mzid
	F221732.dat	Other
	F221732_p0.05.mzid.gz	Mzid

Dataset Information

Surface proteome of emetic Bacillus cereus delta-sl2 delta-eag double mutant strain grown in glucose containing medium

Dataset's files

Publications

Dynamic Profile of S-Layer Proteins Controls Surface Properties of Emetic <i>Bacillus cereus</i> AH187 Strain.

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