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Top-Down proteomics and Bottom-Up terminomics for the Detection of Protein Termini in C. elegans

ABSTRACT: Here we investigate how top-down proteomics (TDP) can be of value for the detection of protein termini. Either GELFrEE or solid-phase enrichment was conducted for pre-fraction. To verify the detected N-termini HUNTER (High-efficiency Undecanal-based N Termini EnRichment) was employed. Reductive dimethylation on intact protein level was additionally applied.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Andreas Tholey

LAB HEAD: Andreas Tholey

PROVIDER: PXD033668 | Pride | 2022-09-26

REPOSITORIES: Pride

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			Action	DRS
	CElegans_MMP_150min_-50-40-30-20_5uL_01.raw	Raw
	CElegans_MMP_150min_-50-40-30-20_5uL_02.raw	Raw
	CElegans_MMP_150min_-50-40-30-20_5uL_03.raw	Raw
	CElegans_MMP_150min_-60-50-40-0_5uL_01.raw	Raw
	CElegans_MMP_150min_-60-50-40-0_5uL_02.raw	Raw

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