Dataset Information

Identifying new interactors of NGN3 through IP-MS

ABSTRACT: NGN3 is a transcription factor whose transient expression during pancreatic development is vital for the generation of endocrine pancreatic cells, including beta cells. NGN3 stabilisation has been shown to induce exocrine-to-endocrine cell plasticity in the murine pancreas, making it a viable target for therapies aiming to replenish beta cells after immune-mediated destruction in type 1 diabetes patients. Here, we set out to identify new interactors of NGN3 that could play a role in its post-translational regulation. We transfected HEK293A cells with HA-tagged NGN3 and carried out immunoprecipitation of the HA-tag, followed by analysis of co-immunoprecipitated interactors via LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

SUBMITTER: Teodora Manea

LAB HEAD: Rocio Sancho

PROVIDER: PXD033691 | Pride | 2023-03-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PR650_TM1_1_NGN_Cntl.mzid.gz	Mzid
	PR650_TM1_1_NGN_Cntl.mzid_PR650_TM1_1_NGN_Cntl.MGF	Mzid
	PR650_TM1_1_NGN_Cntl.raw	Raw
	PR650_TM1_2_NGN_1.mzid.gz	Mzid
	PR650_TM1_2_NGN_1.mzid_PR650_TM1_2_NGN_1.MGF	Mzid

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Publications

USP7 controls NGN3 stability and pancreatic endocrine lineage development.

Manea Teodora T Nelson Jessica Kristine JK Garrone Cristina Maria CM Hansson Karin K Evans Ian I Behrens Axel A Sancho Rocio R

Nature communications 20230428 1

Understanding the factors and mechanisms involved in beta-cell development will guide therapeutic efforts to generate fully functional beta cells for diabetes. Neurogenin 3 (NGN3) is the key transcription factor that marks endocrine progenitors and drives beta-cell differentiation. Here we screen for binding partners of NGN3 and identify the deubiquitylating enzyme USP7 as a key regulator of NGN3 stability. Mechanistically, USP7 interacts with, deubiquitinates and stabilizes NGN3. In vivo, condi ...[more]

PMID: 37117185

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