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Coimmunoprecipitation of BdRFS identifies putative itneracting proteins

ABSTRACT: In this work we sought to identify putative interacting proteins of the Brachypodium distachyon Regulator of Stress and Flowering protein (BdRFS) from healthy leaf tissue. A truncated form of BdRFS lacking the N-terminal 45 residues was necessary for solubilization. THe protein is tagged N-terminally with His-SUMO tag.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Brachypodium Distachyon (purple False Brome) (trachynia Distachya)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Peter Lundquist

LAB HEAD: Peter K. Lundquist

PROVIDER: PXD033835 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Bdistachyon_314_v3.1.protein.fa	Other
	File_List_20220406.xlsx	Xlsx
	MaxQuant_Output_File_-_co-IP_Data.zip	Other
	checksum.txt	Txt
	hf2022041806.raw	Raw

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Publications

A stress-inducible protein regulates drought tolerance and flowering time in Brachypodium and Arabidopsis.

Ying Sheng S Scheible Wolf-Rüdiger WR Lundquist Peter Knut PK

Plant physiology 20230101 1

To cope with environmental stresses and ensure maximal reproductive success, plants have developed strategies to adjust the timing of their transition to reproductive growth. This has a substantial impact on the stress resilience of crops and ultimately on agricultural productivity. Here, we report a previously uncharacterized, plant-specific gene family designated as Regulator of Flowering and Stress (RFS). Overexpression of the BdRFS gene in Brachypodium distachyon delayed flowering, increased ...[more]

PMID: 36264121

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