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Proteomic and long-read transcriptomic analysis of Amblyomma americanum salivary gland lysate

ABSTRACT: Pioneering studies (PXD014844) have identified many interesting molecules in tick saliva by LC-MS/MS proteomics, but the protein databases used to assign mass spectra were based on short Illumina reads of the Amblyomma americanum transcriptome and may not have captured the diversity and complexity of longer transcripts. Here we apply long-read Pacific Bioscience technologies to complement the previously reported short-read Illumina transcriptome-based proteome in an effort to increase spectrum assignments. Our dataset reveals a small increase in assignable spectra to supplement the previously released short-read transcriptome-based proteome.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Amblyomma Americanum

SUBMITTER: Peter Thuy-Boun

LAB HEAD: Seemay Chou

PROVIDER: PXD033870 | Pride | 2022-05-31

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	65	Other
	65.clstr	Other
	AA_Trove_Mulenga_NCBI.fasta	Fasta
	AllPSMs.psmtsv	Other
	AllPeptides.psmtsv	Other

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Time-resolved proteomic profile of Amblyomma americanum tick saliva during feeding Running Title: Proteins in tick saliva every 24 h during feeding

Project description:Pioneering studies (PXD014844) have identified many interesting molecules by LC-MS/MS proteomics, but the protein databases used to assign mass spectra were based on short Illumina reads of the Amblyomma americanum transcriptome and may not have captured the diversity and complexity of longer transcripts. Here we apply long-read Pacific Bioscience technologies to complement the previously reported short-read Illumina transcriptome-based proteome in an effort to increase spectrum assignments. Our dataset reveals a small increase in assignable spectra to supplement previously released short-read transcriptome-based proteome.

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