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Escherichia coli LC-MS/MS treated by tavaborole

ABSTRACT: E.coli ATCC 25922 was overnight grown in 10 ml LB and passaged for 3 h with 1:50 dilution in fresh LB. Treated with tavaborole alone (80 μg/ml, labeled ‘A’) or tavaborole plus amikacin (80 μg/ml +40 μg/ml, labeled ‘AK’) for 6 h, cells were washed in PBS and harvested by centrifugation at 4,000 g, 8 min. After liquid nitrogen flash freezing, cells were stored at -80 °C and transported to company for following experiments

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Suspension Culture

SUBMITTER: Shasha Liu

LAB HEAD: Wu Yong

PROVIDER: PXD033907 | Pride | 2023-03-10

REPOSITORIES: Pride

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Publications

Drug synergy discovery of tavaborole and aminoglycosides against Escherichia coli using high throughput screening.

Liu Shasha S She Pengfei P Li Zehao Z Li Yimin Y Li Linhui L Yang Yifan Y Zhou Linying L Wu Yong Y

AMB Express 20221201 1

High incidences of urinary tract infection (UTI) of aminoglycosides-resistant E.coli causes a severe burden for public health. A new therapeutic strategy to ease this crisis is to repurpose non-antibacterial compounds to increase aminoglycosides sensibility against multidrug resistant E.coli pathogens. Based on high throughput screening technology, we profile the antimicrobial activity of tavaborole, a first antifungal benzoxaborole drug for onychomycosis treatment, and investigate the synergist ...[more]

PMID: 36454354

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