Dataset Information

Proteomics study of antenatal hydronephrosis patients on amniotic fluid

ABSTRACT: In this study, we carried out a comprehensive comparative proteomic and transcriptomic analysis of exosomes in amniotic fluid supernatant and cells in amniotic fluid precipitation of severe fetal hydronephrosis patients to identify candidate biomarkers with diagnostic potential. As the investigation of mechanisms underlying those predicted target genes remain scarce, our studies also provide valuable indications for further researches exploring the role of those genes in fundamental biology or diagnosis of nephrosis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Amniotic Fluid

DISEASE(S): Antenatal Onset,Hydronephrosis

SUBMITTER: Lei Dai

LAB HEAD: Weishe Zhang

PROVIDER: PXD033988 | Pride | 2023-10-24

REPOSITORIES: Pride

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Publications

Multiomics-based study of amniotic fluid small extracellular vesicles identified Moesin as a biomarker for antenatal hydronephrosis.

Li Jingzhi J Fu Ying Y Liu Qiaoshu Q Shen Kuifang K Yao Ruojin R Fu Yimei Y Lu Yang Y Xie Mingkun M Jian Wenyan W Guo Ming M Dai Lei L Zhang Weishe W

Clinical and translational medicine 20230801 8

PMID: 37531302

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Project description:Intra-amniotic infection, the invasion of microbes into the amniotic cavity resulting in an inflammatory process, is a clinical condition that can lead to adverse pregnancy outcomes for the mother and fetus as well as severe long-term neonatal morbidities. Despite much research focused on the consequences of intra-amniotic infection, there is still little knowledge about the functional roles of innate immune cells that respond to invading microbes. In the current study, we performed RNA sequencing of sorted neutrophils and monocytes/macrophages from amniotic fluid from women with intra-amniotic infection to determine the transcriptomic differences between these innate immune cells. Further, we sought to identify specific transcriptomic pathways that were significantly altered by the maternal or fetal origin of amniotic fluid neutrophils and monocytes, the presence of a severe fetal inflammatory response, and pregnancy outcome (i.e. preterm or term delivery). We showed that significant transcriptomic differences exist between amniotic fluid neutrophils and monocytes/macrophages from women with intra-amniotic infection that are indicative of the distinct roles these cells play. We also found that amniotic fluid monocytes/macrophages of fetal origin display impaired ability to clear out microbes invading the amniotic cavity compared to those of maternal origin. Notably, we demonstrate that the transcriptomic changes in amniotic fluid monocytes/macrophages are heavily associated with the severity of the fetal inflammatory response, suggesting that the trafficking of fetal neutrophils throughout the umbilical cord is partially modulated by monocytes/macrophages in the amniotic cavity. Finally, we show that amniotic fluid neutrophils and monocytes/macrophages from preterm deliveries display enhanced transcriptomic activity compared to those from term deliveries, highlighting the protective role of these innate immune cells in this vulnerable period. Collectively, these findings demonstrate the underlying complexity of local innate immune responses in women with intra-amniotic infection, and provide new insights into the functions of amniotic fluid neutrophils and monocytes in the amniotic cavity.

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