Dataset Information

Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays

ABSTRACT: In this study, we developed a model system for the assessment of small molecule-protein interactions using intact cells treated with a commercially available highly specific mitogen-activated protein kinase (MEK) 1/2 inhibitor to prepare a set of unfractionated test samples labeled with tandem mass tags. Our objective was to improve qualitative and quantitative aspects of MS-TSA as well as its efficiency and accuracy. We evaluated individually and for the first time in combination ΦSDM, FAIMS, and SIILCC as an improved MS-based acquisition approach for thermal stability assays (iMAATSA). PSM-level filtering was preliminarily investigated as an approach to reduce melting curve variation and improve the accuracy of Tm measurements.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Cell Culture

SUBMITTER: Clifford Phaneuf

LAB HEAD: Prof. Alexander Ivanov

PROVIDER: PXD034087 | Pride | 2023-05-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CP_Exploris_20200811_DMSO1_NOcarrier_FAIMS_PhiSDM_01.raw	Raw
	CP_Exploris_20200811_DMSO1_NOcarrier_FAIMS_PhiSDM_02.raw	Raw
	CP_Exploris_20200811_DMSO1_NOcarrier_FAIMS_PhiSDM_03.raw	Raw
	CP_Exploris_20200811_DMSO1_NOcarrier_FAIMS_PhiSDM_0_TMT10.pdResult	Other
	CP_Exploris_20200811_DMSO1_NOcarrier_FAIMS_eFT_01.raw	Raw

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Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays.

Phaneuf Clifford G CG Aizikov Konstantin K Grinfeld Dmitry D Kreutzmann Arne A Mourad Daniel D Lange Oliver O Dai Daniel D Zhang Bailin B Belenky Alexei A Makarov Alexander A AA Ivanov Alexander R AR

Communications chemistry 20230406 1

Mass spectrometry (MS)-based thermal stability assays have recently emerged as one of the most promising solutions for the identification of protein-ligand interactions. Here, we have investigated eight combinations of several recently introduced MS-based advancements, including the Phase-Constrained Spectral Deconvolution Method, Field Asymmetric Ion Mobility Spectrometry, and the implementation of a carrier sample as improved MS-based acquisition approaches for thermal stability assays (iMAATS ...[more]

PMID: 37024568

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