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4-HNE modification of human recombinant Dicer

ABSTRACT: We used mass spectrometry analysis (nanoLC-MS/MS) to map 4-HNE-modified residues of human Dicer exposed in vitro to 4-HNE in a concentration-dependent maner

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Alejandro Leyva

LAB HEAD: Rosario Durán

PROVIDER: PXD034113 | Pride | 2023-11-17

REPOSITORIES: Pride

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			Action	DRS
	190430_4-HNE_100__uM_R1.raw	Raw
	190430_4-HNE_100__uM_R2.raw	Raw
	190430_4-HNE_100__uM_R3.raw	Raw
	190430_4-HNE_300__uM_R1.raw	Raw
	190430_4-HNE_300__uM_R2.raw	Raw

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Publications

4-Hydroxynonenal impairs miRNA maturation in heart failure via Dicer post-translational modification.

Kiyuna Ligia A LA Candido Darlan S DS Bechara Luiz R G LRG Jesus Itamar C G ICG Ramalho Lisley S LS Krum Barbara B Albuquerque Ruda P RP Campos Juliane C JC Bozi Luiz H M LHM Zambelli Vanessa O VO Alves Ariane N AN Campolo Nicolás N Mastrogiovanni Mauricio M Bartesaghi Silvina S Leyva Alejandro A Durán Rosario R Radi Rafael R Arantes Guilherme M GM Cunha-Neto Edécio E Mori Marcelo A MA Chen Che-Hong CH Yang Wenjin W Mochly-Rosen Daria D MacRae Ian J IJ Ferreira Ludmila R P LRP Ferreira Julio C B JCB

European heart journal 20231101 44

<h4>Background and aims</h4>Developing novel therapies to battle the global public health burden of heart failure remains challenging. This study investigates the underlying mechanisms and potential treatment for 4-hydroxynonenal (4-HNE) deleterious effects in heart failure.<h4>Methods</h4>Biochemical, functional, and histochemical measurements were applied to identify 4-HNE adducts in rat and human failing hearts. In vitro studies were performed to validate 4-HNE targets.<h4>Results</h4>4-HNE, ...[more]

PMID: 37944136

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