A multi¬omic analysis reveal gender differences in the hippocampus of Alzheimer disease subjects

ABSTRACT: Alzheimer’s disease (AD) is the most common neurodegenerative pathology affecting more than 20 million people worldwide and the leading cause of dementia. Although AD neuropathology has been well defined, the underlying causes of the disease remain debatable. Recently, sex and gender are emerging as crucial drivers of development and progression of AD dementia. So, to investigate the molecular basis of gender¬related influences on the pathology of AD, we analyzed hippocampal brain tissues from control (CTR) and Alzheimer’s Disease (AD) patients using an integrated proteomic, metabolomics and transcriptomic approach. A principal component analysis carried out to identify major differences among the two datasets highlights differences among AD and CTR. Focusing on the gender dependent omic changes, comparative analyses were carried out considering males (M) and females (F) as separate subgroups in AD patients and in healthy subjects. The analysis was conducted considering proteins differentially expressed if they were present only in one condition or showed significant t¬test difference. The results strongly suggest that there is a significant difference between male and female in all the omic data sets either in the neurodegenerative disease and in the control. Preliminary results from bioinformatics analysis highlight major differences in energetic metabolism, cytoskeleton organization and oxidative stress response.The results are of main relevance for future clinical interventions that can reduce dementia risk in both male and female patients.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Francesca Grassi Scalvini  

LAB HEAD: Francesca Grassi Scalvini

PROVIDER: PXD034137 | Pride | 2024-10-01

REPOSITORIES: Pride