Dataset Information

Pathogen protein modularity enables mimicry of a host phosphatase

ABSTRACT: Pathogens rely on a diverse complement of effector proteins to manipulate host cellular processes. The devastating plant pathogen Phytophthora produces numerous effectors that consist of tandem repeats of the “(L)WY” motif. Here, we discovered a specific (L)WY-LWY combination that enables efficient recruitment of the Serine/Threonine protein phosphatase 2A (PP2A) core enzyme in plant hosts. Structural characterization of an effector-PP2A complex and biochemical analyses demonstrate multiple effectors adopt this module at the amino terminus to form functional PP2A holoenzymes but have divergent C-terminal LWY units. Our results discovered a virulence strategy widely employed by Phytophthora in which an essential phosphatase complex of the host is appropriated to promote disease and highlight how diversification in an effector repertoire is promoted by protein modularity.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Jan Sklenar

LAB HEAD: Wenbo Ma

PROVIDER: PXD034179 | Pride | 2024-10-01

REPOSITORIES: pride

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			Action	DRS
	checksum.txt	Txt
	hli210520_15142-1.raw	Raw
	hli210520_15142-1__F155479_.mzid.gz	Mzid
	hli210520_15142-1__F155479_.mzid_hli210520_15142-1__F155479_.MGF	Mzid
	hli210520_15142-2.raw	Raw

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Publications

Pathogen protein modularity enables elaborate mimicry of a host phosphatase.

Li Hui H Wang Jinlong J Kuan Tung Ariel TA Tang Bozeng B Feng Li L Wang Jiuyu J Cheng Zhi Z Skłenar Jan J Derbyshire Paul P Hulin Michelle M Li Yufei Y Zhai Yi Y Hou Yingnan Y Menke Frank L H FLH Wang Yanli Y Ma Wenbo W

Cell 20230626 15

Pathogens produce diverse effector proteins to manipulate host cellular processes. However, how functional diversity is generated in an effector repertoire is poorly understood. Many effectors in the devastating plant pathogen Phytophthora contain tandem repeats of the "(L)WY" motif, which are structurally conserved but variable in sequences. Here, we discovered a functional module formed by a specific (L)WY-LWY combination in multiple Phytophthora effectors, which efficiently recruits the serin ...[more]

PMID: 37369204

Dataset Information

Pathogen protein modularity enables mimicry of a host phosphatase

Dataset's files

Publications

Pathogen protein modularity enables elaborate mimicry of a host phosphatase.

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