Proteomics

Dataset Information

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Pulldown of proteins interacting with SGOL1-AS1 in AML

ABSTRACT: Epigenetic dysregulation is a common feature of acute myeloid leukemia (AML). Recently it has become clear that long noncoding RNAs (lncRNAs) can play a key role in epigenetic regulation, and consequently also dysregulation. Currently, our understanding of the requirements and roles of lncRNAs in AML is still limited. Using CRISPRi screening, we identified the lncRNA SGOL1-AS1 as an essential regulator of survival in THP-1 AML cells. We use RNA affinity purification using a biotinylated bait to pull down binding partners of the lncRNA, SGOL1-AS1. The identified proteins show a signficant enrichment for chromatin-modifying proteins involved in gene repression and chromosome organization.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood

DISEASE(S): Acute Leukemia

SUBMITTER: Anna Hoyle  

LAB HEAD: Georges Lacaud

PROVIDER: PXD034329 | Pride | 2023-06-12

REPOSITORIES: Pride

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081221_ES_54410_antisense_a.raw Raw
081221_ES_54410_antisense_b.raw Raw
081221_ES_54410_antisense_c.raw Raw
081221_ES_54410_antisense_d.raw Raw
081221_ES_54410_sense_a.raw Raw
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Publications

SGOL1-AS1 enhances cell survival in acute myeloid leukemia by maintaining pro-inflammatory signaling.

Selkirk Ewan E   Patel Rahima R   Hoyle Anna A   Lie-A-Ling Michael M   Smith Duncan D   Swift Joe J   Lacaud Georges G  

Heliyon 20221105 11

Epigenetic dysregulation is a key feature of most acute myeloid leukemia (AML). Recently, it has become clear that long noncoding RNAs (lncRNAs) can play a key role in epigenetic regulation, and consequently also dysregulation. Currently, our understanding of the requirements and roles of lncRNAs in AML is still limited. Here, using CRISPRi screening, we identified the lncRNA SGOL1-AS1 as an essential regulator of survival in THP-1 AML cells. We demonstrated that SGOL1-AS1 interacts with chromat  ...[more]

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