Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Venom dynamics across populations of Nematostella

ABSTRACT: Here we perform semi quantitative analysist of the proteome of Nematostella vectensis from different locations in the USA to compare abundance of different toxins

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Nematostella Vectensis

TISSUE(S): Whole Body

SUBMITTER: joachim surm

LAB HEAD: Yehu Moran

PROVIDER: PXD034383 | Pride | 2022-12-21

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Book3.xml	Xml
	FL1.raw	Raw
	FL2.raw	Raw
	FL3.raw	Raw
	FL4.raw	Raw

Items per page:

1 - 5 of 10

Similar Datasets

Venom tradeoff shapes interspecific interactions, physiology and reproduction

Project description:An animal’s ability to effectively capture prey and defend against predators is pivotal to its survival1. A key innovation in many predator-prey interactions is venom, which consists of many toxin proteins that shape its phenotype2. Its unusually direct relationship of gene-toxin-phenotype make it an appealing system for studies at the organismal level 3,4. In this work we use the sea anemone Nematostella vectensis as a model organism as it provides us with the opportunity to test for the first time how toxin-genotypes impact predator-prey interactions. Specifically, we compare a native-population5 and a transgenic line which both have significantly reduced levels of Nv1, the major toxin in adult Nematostella6, to animals with wildtype levels of Nv1. We demonstrate that the transgenic strain phenocopies native anemones lacking Nv1 as they both have impaired ability to defend themselves against grass shrimp, a native predator. Mummichog killifish, unexpectedly are attracted to Nematostella with wildtype-levels of Nv1, highlighting that Nv1 plays a complex role in shaping interspecific-interactions. Finally, we demonstrate an evolutionary tradeoff as the reduction of Nv1 levels causes faster growth and increased reproductive rates compared to Nematostella control lines. Overall, our results experimentally link organism’s venom to its physiology, reproduction and interspecific interactions.

2023-12-31 | PXD043484 | Pride

Geographical temperature stress in the sea anemone Nematostella vectensis

Project description:Gene expression of Nematostella vectensis from 5 different locations under chronic (30 day) temperature stress

2020-01-24 | GSE144104 | GEO

Venom tradeoff shapes interspecific interactions, physiology and reproduction

2023-12-31 | PXD043447 | Pride

Nematostella vectensis argonautes

Project description:We performed Immuno precipitation using antibodies against Nematostella vectensis Argonauts proteins (i.e AGO1 and AGO2). Samples of the IP were analyzed by LC-MSMS in order to idetify the argonautes and prove antibodies specifity.

2020-10-14 | PXD011644 | Pride

Nematostella vectensis

Project description:Nematostella vectensis RAD sequencing

| PRJNA170681 | ENA

Nematostella vectensis

Project description:Nematostella vectensis Genome sequencing

| PRJNA337090 | ENA

Impact of antibiotics, cell-free supernatant, LPS, and live cells on Nematostella vectensis gene expression

Project description:We used bacteria isolated from field samples of Nematostella vectensis to quantify gene expression through comparisons of transcriptomes.

2021-02-20 | GSE167114 | GEO

Nematostella toxins expression under stress

Project description:Changes in Nematostella vectensis proteome expression were analyzed in 2 different Nematostella populations along the east coast of USA in different stress conditions vrs. normal growth temperature.

2020-08-05 | PXD016943 | Pride

Nematostella vectensis

Project description:Bacterial interactions in Nematostella vectensis

| PRJNA433067 | ENA

Nematostella vectensis

Project description:Nematostella vectensis Raw sequence reads

| PRJNA521074 | ENA