Proteomics

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Evaluation of the potential risk of Easy-nLC 1200 in phosphoproteomics

ABSTRACT: Liquid chromatography-mass spectrometry (LC-MS) is a major tool for large scale qualitative and/or quantitative analysis of protein phosphorylation in cells or tissues. Performance of LC is pivotal for the success of phosphoprotemics in both sensitivity and reproducibility. Here we report that the widely used Easy-nLC 1200 has poor performance in analyzing phosphopeptides, particularly in terms of sensitivity and reproducibility, whereas its predecessor, Easy-nLC 1000 has much better performance.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human) Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Epithelial Cell

SUBMITTER: Yuanya Zhang  

LAB HEAD: Yingchun Wang

PROVIDER: PXD034396 | Pride | 2023-03-10

REPOSITORIES: Pride

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Elite_EY1000_20210727_293T_PHOS_200ug_1.raw Raw
Elite_EY1000_20210727_293T_PHOS_200ug_2.raw Raw
Elite_EY1000_20210727_293T_PHOS_200ug_3.raw Raw
Elite_EY1000_20210727_293T_PHOS_600ug_1.raw Raw
Elite_EY1000_20210727_293T_PHOS_600ug_2.raw Raw
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Publications

Evaluation of the Potential Risk of Analyzing Phosphopeptides with Easy-nLC 1200-Coupled Mass Spectrometers.

Duan Xiaoxiao X   Zhang Yuanya Y   Huang Xiahe X   Ma Xiao X   Gao Hui H   Wang Yingchun Y  

ACS omega 20221214 51

Liquid chromatography-mass spectrometry (LC-MS) is a major tool for the large-scale qualitative and/or quantitative analysis of protein phosphorylation in cells or tissues. The performance of LC is pivotal for the success of phosphoproteomics in both sensitivity and reproducibility. Here, we report that the widely used Easy-nLC 1200 has poor performance in analyzing phosphopeptides, particularly in terms of sensitivity and reproducibility, whereas its predecessor, Easy-nLC 1000, has a much bette  ...[more]

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