Dataset Information

Proteomic Analysis of RAP (RNA Antisense Purification) isolated RNPs (RiboNucleotide Particles) harboring the novel lncRNA mimi

ABSTRACT: In neurons, mRNAs and associated RNA-binding proteins assemble into ribonucleoprotein (RNP) granules essential to regulate mRNA trafficking, local translation, and turnover. Dysregulation of RNA-protein condensation can disturb synaptic plasticity. We report that the novel lncRNA mimi is a constitutive and essential component of large cytoplasmic condensates (RNP granules) in fly neurons. In order to identify direct mimi RNA binders we employ RAP assisted purification of mimi RNPs subsequent to UV-crosslinking of adult fly brain tissue (non UV irradiated samples serve as control). Applying relative Max Quant LFQ quantification (Max LFQ) we carry out a differential proteomic analysis of mimi RNP complexes in UV-irradiated versus non irradiated fly brains.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Brain

SUBMITTER: Gerhard Mittler

LAB HEAD: Gerhard Mittler

PROVIDER: PXD034457 | Pride | 2022-10-12

REPOSITORIES: Pride

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The long noncoding RNA <i>mimi</i> scaffolds neuronal granules to maintain nervous system maturity.

Grzejda Dominika D Mach Jana J Schweizer Johanna Aurelia JA Hummel Barbara B Rezansoff Andrew Mischa AM Eggenhofer Florian F Panhale Amol A Lalioti Maria-Eleni ME Cabezas Wallscheid Nina N Backofen Rolf R Felsenberg Johannes J Hilgers Valérie V

Science advances 20220928 39

RNA binding proteins and messenger RNAs (mRNAs) assemble into ribonucleoprotein granules that regulate mRNA trafficking, local translation, and turnover. The dysregulation of RNA-protein condensation disturbs synaptic plasticity and neuron survival and has been widely associated with human neurological disease. Neuronal granules are thought to condense around particular proteins that dictate the identity and composition of each granule type. Here, we show in <i>Drosophila</i> that a previously u ...[more]

PMID: 36170367

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