Dataset Information

Organism-wide secretome mapping of tissue crosstalk in exercise

ABSTRACT: Physical activity is a powerful physiologic stimulus that provides benefits to multiple organ systems and confers protection against disease. These physiologic effects are in part mediated by blood-borne factors that mediate tissue crosstalk and function as molecular effectors of physical activity. To globally understand how physical activity reshapes cellular secretomes, here we applied a proximity biotinylation approach to profile cell type-specific secretomes from blood plasma following treadmill running in mice. This organism-wide, 21-cell type, 10-tissue in-vivo secretome atlas reveals complex cell type-specific and bidirectional modulation of secreted proteins across all cell types following exercise training. We identify a gradient of secretome responses across cell types, with secretomes from Pdgfra+ being one of the most exercise-responsive in the entire dataset. Peptide-level correlation analysis revealed widespread and cell type-specific exercise regulation of secreted proteoforms. Finally, we show that exercise-inducible secretion of soluble CES2 proteins from the liver suppresses obesity in high fat diet-fed mouse models. Together, these data identify specific exercise-regulated cell types and secreted proteins and illuminate the dynamic remodeling of cell and tissue crosstalk by physical activity.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Plasma

SUBMITTER: Nicholas Riley

LAB HEAD: Jonathan Long

PROVIDER: PXD034535 | Pride | 2023-07-21

REPOSITORIES: Pride

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Organism-wide, cell-type-specific secretome mapping of exercise training in mice.

Wei Wei W Riley Nicholas M NM Lyu Xuchao X Shen Xiaotao X Guo Jing J Raun Steffen H SH Zhao Meng M Moya-Garzon Maria Dolores MD Basu Himanish H Sheng-Hwa Tung Alan A Li Veronica L VL Huang Wentao W Wiggenhorn Amanda L AL Svensson Katrin J KJ Snyder Michael P MP Bertozzi Carolyn R CR Long Jonathan Z JZ

Cell metabolism 20230503 7

There is a significant interest in identifying blood-borne factors that mediate tissue crosstalk and function as molecular effectors of physical activity. Although past studies have focused on an individual molecule or cell type, the organism-wide secretome response to physical activity has not been evaluated. Here, we use a cell-type-specific proteomic approach to generate a 21-cell-type, 10-tissue map of exercise training-regulated secretomes in mice. Our dataset identifies >200 exercise train ...[more]

PMID: 37141889

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	AdipoqCreRun1.raw	Raw
	AdipoqCreRun2.raw	Raw
	AdipoqCreRun3.raw	Raw
	AdipoqCreSed1.raw	Raw
	AdipoqCreSed2.raw	Raw