Proteomics

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Profiling of mouse brain acetylome and proteome

ABSTRACT: Nε-Lysine acetylation is a reversible post-translational protein modification (PTM). In recent years, genetic analysis and animal model studies have shown that protein acetylation is closely related to the development of the nervous system and neurodegenerative diseases such as PD and AD. Here, we aim to profile the mouse brain acetylome by using FSAP, immunoaffinity precipitation of acetylpeptides and mass spectrometry method. We identified 1,591 acetylated proteins containing 4,182 acetylation modification sites in C57BL/6 mice brain. These proteins were involved in myelin sheath, mitochondrial inner membrane and neuron synapse. While KEGG enrichment analysis indicated that these proteins are associated with neurological diseases, such as Alzheimer's disease and Huntington disease. Taken together, we provided a comprehensive profile and clues for understanding the role of acetylated proteins in neuropathology such as neurogenesis, degeneration and aging.

INSTRUMENT(S): Orbitrap Fusion Lumos, TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Yuhua JI  

LAB HEAD: Yuhua Ji

PROVIDER: PXD034594 | Pride | 2022-10-14

REPOSITORIES: Pride

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20170711_BR.pdResult Other
20170711_BR.raw Raw
BR1.raw Raw
Brain_T.wiff Wiff
Brain_T.wiff.scan Wiff
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Publications

A comprehensive mouse brain acetylome-the cellular-specific distribution of acetylated brain proteins.

Ji Yuhua Y   Chen Zixin Z   Cen Ziqi Z   Ye Yuting Y   Li Shuyuan S   Lu Xiaoshuang X   Shao Qian Q   Wang Donghao D   Ji Juling J   Ji Qiuhong Q  

Frontiers in cellular neuroscience 20220830

Nε-lysine acetylation is a reversible posttranslational modification (PTM) involved in multiple physiological functions. Genetic and animal studies have documented the critical roles of protein acetylation in brain development, functions, and various neurological disorders. However, the underlying cellular and molecular mechanism are still partially understood. Here, we profiled and characterized the mouse brain acetylome and investigated the cellular distribution of acetylated brain proteins. W  ...[more]

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