Proteomics

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Short-term administration of common anesthetics does not dramatically change the endogenous peptide profile in the rat pituitary


ABSTRACT: To identify and quantify the relative abundances of cell-cell signaling peptides in different physiological states, LC-MS-based peptidomics workflows are commonly utilized on freshly dissected tissue. In such animal experiments, the administration of general anesthetics is an important step for many research projects. However, acute anesthetic administration may rapidly change the measured abundance of transmitter molecules and metabolites which may confound experimental results. The aim of this study was to evaluate the effect of short-term (< 5 min) anesthetic administration on the measured abundance pituitary peptides, as evaluated by a typical peptidomics workflow. To accomplish this goal, we compared peptide abundances in the rat pituitary following administration of 5% isoflurane, 200 mg/kg sodium pentobarbital, or no anesthetic administration using a label-free peptidomics protocol.

INSTRUMENT(S): Xevo G2-XS QTof

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Hypophysis

SUBMITTER: James Checco  

LAB HEAD: James Checco

PROVIDER: PXD034821 | Pride | 2022-09-23

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20211105_SM0837_Pituitary_02.mgf Mgf
20211105_SM0837_Pituitary_02.raw.rar Raw
20211105_SM0837_Pituitary_04.mgf Mgf
20211105_SM0837_Pituitary_04.raw.rar Raw
20211105_SM0837_Pituitary_06.mgf Mgf
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