Dataset Information

Rat sensory and motor nerve fibroblasts LC-MSMS

ABSTRACT: In this study, a quantitative proteomic technique based on isobaric tags for relative and absolute quantitation (iTRAQ) was used to compare the proteome of cultured sensory and motor nerve fibroblasts (Fbs). Among a total of 2597 overlapping proteins identified, we obtained 148 differentially expressed proteins, of which 116 proteins were significantly higher expressed in sensory Fbs, and 32 proteins were significantly higher expressed in motor Fbs. Western blot and qPCR analysis were applied to validate differentially expressed proteins. Functional categorization indicated that differentially expressed proteins were linked to a diverse array of biological processes , including regeneration, axon guidance, cytoskeleton organization, cell proliferation, cell migration, cell adhesion, and tissue remodeling, which might play a critical role in the specificity of peripheral nerve regeneration. Furthermore, following co-culture of motor neurons with motor or sensory Fbs , motor Fbs significantly enhanced neurite growth than sensory Fbs. These findings indicated that nerve Fbs expressed the distinct motor and sensory phenotypes involved in different patterns of protein expression, biological processes, and effects on neurons.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Rattus Norvegicus (rat)

SUBMITTER: Yuhua JI

LAB HEAD: Yuhua Ji

PROVIDER: PXD034827 | Pride | 2023-03-11

REPOSITORIES: Pride

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Sensory and motor fibroblasts have different protein expression patterns and exert different growth promoting effects on sensory and motor neurons.

He Qianru Q Cheng Zhenghang Z Zhou Qiang Q Tong Fang F Li Yan Y Zhou Xinyang X Yu Miaomei M Ji Yuhua Y Ding Fei F

Experimental neurology 20221228

Functional reconstruction after peripheral nerve injury depends on the ability of the regenerated sensory and motor axons to re-innervate the suitable target organs. Therefore, it is essential to explore the cellular mechanisms of peripheral nerve-specific regeneration. In a previous study, we found that sensory and motor fibroblasts can guide Schwann cells to migrate towards the same phenotype. In the present paper, we analyzed the different effects of sensory and motor fibroblasts on sensory o ...[more]

PMID: 36586550

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