Proteomics

Dataset Information

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A comprehensive and quantitative interactome of RAS


ABSTRACT: We investigated a comprehensive and quantitative interactome of RAS, a protein found to be a driver of many human cancers. This resource identifies interactors of the active form of RAS (nucleotide-dependent) as well as isoform-specific (KRAS, HRAS, and NRAS) interactors of RAS. Several of the proteins identified were confirmed as being important for cancer cell viability and senescence.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Sheng Zhang  

LAB HEAD: Sheng Zhang

PROVIDER: PXD034847 | Pride | 2022-10-13

REPOSITORIES: pride

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Publications

A Proteomic Approach Identifies Isoform-Specific and Nucleotide-Dependent RAS Interactions.

Miller Seth P SP   Maio George G   Zhang Xiaoyu X   Badillo Soto Felix S FS   Zhu Julia J   Ramirez Stephen Z SZ   Lin Hening H  

Molecular & cellular proteomics : MCP 20220714 8


Active mutations in the RAS genes are found in ∼30% of human cancers. Although thought to have overlapping functions, RAS isoforms show preferential activation in human tumors, which prompted us to employ a comparative and quantitative proteomics approach to generate isoform-specific and nucleotide-dependent interactomes of the four RAS isoforms, KRAS4A, KRAS4B, HRAS, and NRAS. Many isoform-specific interacting proteins were identified, including HRAS-specific CARM1 and CHK1 and KRAS-specific PI  ...[more]

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