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TMT proteomic analysis of tropical quinoa (Chenopodium quinoa willd.) seeds during maturation

ABSTRACT: Many limitation factors, such as seed pre-harvest sprouting, bitter saponin e.c., greatly restricts quinoa production and popularity. To solves these problems, the underlying mechanism of seed maturation in quinoa was required. In this study, morphological observation, TMT proteomics and parallel reaction monitoring (PRM) were conducted to investigate the variation of quinoa seed during seed maturation

INSTRUMENT(S): Q Exactive

ORGANISM(S): Chenopodium Quinoa

TISSUE(S): Plant Cell, Embryo

SUBMITTER: Zhi-Jun Shen

LAB HEAD: Zhi-Jun Shen

PROVIDER: PXD034885 | Pride | 2022-10-19

REPOSITORIES: Pride

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	Raw_data.zip	Other
	allPeptides.txt	Txt

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Chenopodium quinoa

Project description:Quinoa seed Transcriptome

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Project description:Quinoa seed Transcriptome

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Next-Generation Sequencing Facilitates Quantitative Analysis of Two Kinds of Chenopodium quinoa Transcriptomes

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Genome-wide analysis of CqCrRLK1Ls and CqRALFs in Chenopodium quinoa

Project description:Quinoa (Chenopodium quinoa) is a highly nutritious crop showing a remarkable tolerance to multiple abiotic stresses. The Catharanthus roseus RLK1-like kinase (CrRLK1L) family is involved in multiple processes during plant growth and stress responses. However, little is known about the members of the CqCrRLK1L subfamily and their biological functions. In this study, up to 26 CqCrRLK1L members were identified in quinoa genome. To systematically investigate their tissue-specific expression pattern and gene expression profiles in response to abiotic stresses, different quinoa samples including leaf, pistil, stamen, root, and leaf treated with NaCl were harvested for transcrioptome analysis with three biological replicates for each sample. Finally,we mapped about 30 million sequence reads per sample to the quinoa genome (NL-6) and combining the four different tissues (leaf, pistil, stamen, and root), totally 40382 genes were detected, accounting for 69% of total genes in quinoa. The expression of most CqCrRLK1Ls genes were detected in all four kind of tissues, and they showed tissue-specific expression pattern. In salt-treated leaves, we observe that about 6-thosands genes changed their expression, with log2FoldChange < -1 or >1 and p value <0.05. And four CqCrRLK1L genes largely altered their transcript levels, implying that these genes are involved in the regulation of salt stress response in quinoa. The present study provides a base for future research on elucidating the varied biological functions of CqCrRLK1Ls and their contributions to stress responses.

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RNAseq of Quinoa salt bladders vs. leaves

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