Dataset Information

Prtg+ve Rhombic Lip Ventricular Zone Stem Cells Initiate and Maintain Group 3 Medulloblastoma

ABSTRACT: PRTG+ve cells show high self renewability in Group 3 medulloblastoma tumors. To access the proteins differentially expressed in this subset of cells, we sorted PRTG+ve and PRTG-ve cells by surface staining with Anti-PRTG antibody from Gr3 medulloblastoma xenografts.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

DISEASE(S): Brain Cancer

SUBMITTER: Abhirami Visvanathan

LAB HEAD: Dr. Michael D. Taylor

PROVIDER: PXD034890 | Pride | 2024-07-31

REPOSITORIES: Pride

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Early rhombic lip Protogenin+ve stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma.

Visvanathan Abhirami A Saulnier Olivier O Chen Chuan C Haldipur Parthiv P Orisme Wilda W Delaidelli Alberto A Shin Seungmin S Millman Jake J Bryant Andrew A Abeysundara Namal N Wu Xujia X Hendrikse Liam D LD Patil Vikas V Bashardanesh Zahedeh Z Golser Joseph J Livingston Bryn G BG Nakashima Takuma T Funakoshi Yusuke Y Ong Winnie W Rasnitsyn Alexandra A Aldinger Kimberly A KA Richman Cory M CM Van Ommeren Randy R Lee John J Y JJY Ly Michelle M Vladoiu Maria C MC Kharas Kaitlin K Balin Polina P Erickson Anders W AW Fong Vernon V Zhang Jiao J Suárez Raúl A RA Wang Hao H Huang Ning N Pallota Jonelle G JG Douglas Tajana T Haapasalo Joonas J Razavi Ferechte F Silvestri Evelina E Sirbu Olga O Worme Samantha S Kameda-Smith Michelle M MM Wu Xiaochong X Daniels Craig C MichaelRaj Antony K AK Bhaduri Aparna A Schramek Daniel D Suzuki Hiromichi H Garzia Livia L Ahmed Nabil N Kleinman Claudia L CL Stein Lincoln D LD Dirks Peter P Dunham Christopher C Jabado Nada N Rich Jeremy N JN Li Wei W Sorensen Poul H PH Wechsler-Reya Robert J RJ Weiss William A WA Millen Kathleen J KJ Ellison David W DW Dimitrov Dimiter S DS Taylor Michael D MD

Cell 20240705 17

We identify a population of Protogenin-positive (PRTG+ve) MYChigh NESTINlow stem cells in the four-week-old human embryonic hindbrain that subsequently localizes to the ventricular zone of the rhombic lip (RLVZ). Oncogenic transformation of early Prtg+ve rhombic lip stem cells initiates group 3 medulloblastoma (Gr3-MB)-like tumors. PRTG+ve stem cells grow adjacent to a human-specific interposed vascular plexus in the RLVZ</su ...[more]

PMID: 38971152

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Project description:Neurospora crassa is a reference organism to study carotene biosynthesis and light regulation for decades. However, there is no evidence of its capacity to produce secondary metabolites, a characteristic of many filamentous fungi. In this work, we report the role of the fungal specific velvet regulator complex in development and secondary metabolism in N. crassa. Four velvet genes were found in the genome. Deletion of ve-1 or ve-2 affects asexual and sexual development, secondary metabolism and light-dependent carotene biosynthesis. Deletion of vos-1 did not show significant differences in comparison to wild type. Deletion of lae-1 resulted in reduced protoperithecia formation and affected secondary metabolism. VE-1, VE-2, LAE-1 and VOS-1 showed nucleo-cytoplasmic localization, which was independent of light input. Two distinct velvet complexes were found in vivo: a heterotrimeric VE-1/VE-2/LAE-1 and a heterodimeric VE-2/VOS-1 complex, respectively. The heterotrimer-complex positively regulated sexual development and repressed asexual spore formation. Moreover, it repressed siderophore coprogen production under iron starvation conditions. The VE-1/VE-2 heterodimer controlled the production of carotene pigments. VE-1 regulated the expression of more than 15% of the whole genome, which corresponded mainly to regulatory, developmental and redox proteins. We also studied intergenera functions of the velvet complex through complementation of A. nidulans veA, velB, laeA, vosA mutants with their Neurospora crassa orthologues ve-1, ve-2, lae-1 and vos-1, respectively. Expression of VE-1 and VE-2 in A. nidulans successfully substituted the developmental and secondary metabolite functions of VeA and VelB by forming two functional chimeric velvet complexes in vivo, VelB/VE-1/LaeA and VE-2/VeA/LaeA, respectively. The N. crassa lae-1 and vos-1 genes did not complement respective A. nidulans mutants and failed to form corresponding complexes. Reciprocally, expression of veA restored the phenotypes of the N. crassa ve-1 mutant. All N. crassa velvet proteins heterologously expressed in A. nidulans were predominantly localized to nuclear fraction independent of light signal. These data highlight the conservation of the complex formation potential in N. crassa and A. nidulans. However, they also underline the similarities and differences of the velvet roles across genera according to the different life styles, niches and ontogenetic processes.

			Action	DRS
	051_PRTG_Neg.raw	Raw
	051_PRTG_Pos.raw	Raw
	20210601_LFQ_Results.msf	Msf
	20210611_LFQ_Results.msf	Msf
	211_PRTG_Neg.raw	Raw

Dataset Information

Prtg+ve Rhombic Lip Ventricular Zone Stem Cells Initiate and Maintain Group 3 Medulloblastoma

Dataset's files

Publications

Early rhombic lip Protogenin<sup>+ve</sup> stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma.

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Dataset Information

Prtg+ve Rhombic Lip Ventricular Zone Stem Cells Initiate and Maintain Group 3 Medulloblastoma

Dataset's files

Publications

Early rhombic lip Protogenin&lt;sup&gt;+ve&lt;/sup&gt; stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Early rhombic lip Protogenin<sup>+ve</sup> stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma.