Proteomics

Dataset Information

0

Impact of immunoproteasome impairment on microglia cell function


ABSTRACT: The ubiquitin-proteasome system maintains the functional proteome of the cells by the clearance of damaged, misfolded, old and/or unneeded proteins. This is particularly important in the brain where protein accumulation has a hallmark of many neurodegenerative diseases that drives neuroinflammation. Microglia are the resident immune cells of the central nervous system and play a major role in the regulation of brain homeostasis via constitutive expression of standard proteasomes and immunoproteasomes (IP). Nevertheless, the impact of IP function on the innate immunity of CNS is not well described. Here, we analyzed ubiquitylated proteins in IP deficient microglia upon enrichment and under different conditions to identify the proteins preferentially degraded by the IP and to investigate the impact of the accumulation of these proteins on microglia function.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Cell Culture, Microglial Cell

SUBMITTER: Simone Venz  

LAB HEAD: Simone Venz

PROVIDER: PXD034900 | Pride | 2023-03-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20200429_OV1_SV_BCH_SV_GC_R1_1.msf Msf
20200429_OV1_SV_BCH_SV_GC_R1_1.raw Raw
20200429_OV1_SV_BCH_SV_GC_R1_2.msf Msf
20200429_OV1_SV_BCH_SV_GC_R1_2.raw Raw
20200429_OV1_SV_BCH_SV_GC_R1_3.msf Msf
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Publications

Immunoproteasomes control activation of innate immune signaling and microglial function.

Çetin Gonca G   Studencka-Turski Maja M   Venz Simone S   Schormann Eileen E   Junker Heike H   Hammer Elke E   Völker Uwe U   Ebstein Frédéric F   Krüger Elke E  

Frontiers in immunology 20221006


Microglia are the resident immune cells of the central nervous system (CNS) and play a major role in the regulation of brain homeostasis. To maintain their cellular protein homeostasis, microglia express standard proteasomes and immunoproteasomes (IP), a proteasome isoform that preserves protein homeostasis also in non-immune cells under challenging conditions. The impact of IP on microglia function in innate immunity of the CNS is however not well described. Here, we establish that IP impairmen  ...[more]

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