Proteomics

Dataset Information

0

Chaperonin CCT controls cell fuelling by lipids and extracellular vesicle production through kinesin dynamics


ABSTRACT: Please provide an overall description of your study, think something similar in scope to the manuscript abstractCell proteostasiCell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modification, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganellar contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.s includes gene transcription, protein translation, folding of de novo proteins, post-translational modification, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganellar contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Enrique Calvo  

LAB HEAD: Enrique Calvo

PROVIDER: PXD034917 | Pride | 2023-07-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
cells_siCCT_1.msf Msf
cells_siCCT_1.raw Raw
cells_siCCT_2.msf Msf
cells_siCCT_2.raw Raw
cells_siCCT_3.msf Msf
Items per page:
1 - 5 of 24
altmetric image

Publications


Cell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modifications, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activi  ...[more]

Similar Datasets

2015-03-23 | GSE59930 | GEO
2022-12-05 | PXD037474 | Pride
2017-03-23 | PXD005123 | Pride
2017-11-01 | GSE95489 | GEO
2021-09-09 | PXD018834 | Pride
2014-04-15 | E-GEOD-56783 | biostudies-arrayexpress
2014-04-15 | GSE56783 | GEO
2016-06-28 | E-GEOD-75247 | biostudies-arrayexpress
2016-06-28 | E-GEOD-75410 | biostudies-arrayexpress
2019-10-15 | GSE132298 | GEO