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Local burn wound environment verses systemic response: comparison of proteins and metabolites

ABSTRACT: In this study, paired blood plasma (BP) and blister fluid (BF) samples from 5 paediatric burn patients were analysed using mass spectrometry to compare their protein and metabolite composition. The relative quantification of proteins was achieved through a label-free data independent acquisition mode. The relative quantification of metabolites was achieved using a Shimadzu Smart Metabolite Database gas chromatography mass spectrometry (GCMS) targeted assay. In total, 562 proteins and 141 individual metabolites were identified in the samples. There was 81% similarity in the proteins present in the BP and BF, with 50 and 54 unique proteins found in each sample type respectively. BF contained keratinocyte proliferation-related proteins and blood plasma contained abundant blood clotting proteins and apolipoproteins. BF contained more carbohydrates and less alpha-hydroxy acid metabolites than the BP. In this study, there were unique proteins and metabolites in BF and BP which were reflective of the local wound environment and systemic environments respectively. The results from this study demonstrate that the biomolecule content of BF is mostly the same as blood, but it also contains information specific to the local wound environment.

INSTRUMENT(S): TripleTOF 5600, Shimadzu instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma, Wound Fluid

DISEASE(S): Wounds And Injuries

SUBMITTER: Tuo Zang

LAB HEAD: Leila Cuttle

PROVIDER: PXD034978 | Pride | 2022-08-15

REPOSITORIES: Pride

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			Action	DRS
	202101010D_SWATH_L433.wiff	Wiff
	202101010D_SWATH_L433.wiff.scan	Wiff
	202101010D_SWATH_L434.wiff	Wiff
	202101010D_SWATH_L434.wiff.scan	Wiff
	202101010D_SWATH_L435.wiff	Wiff

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