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Contraction of brain pericytes in response to Islet Amyloid PolyPeptide is reversed by pramlintide.

ABSTRACT: The islet amyloid polypeptide (IAPP), a pancreas-produced peptide, has beneficial functions in its monomeric form. However, IAPP aggregates, related to type 2 diabetes mellitus (T2DM), are toxic not only for the pancreas, but also for the brain. In the latter, IAPP is often found in vessels, where it is highly toxic for pericytes, cells that have contractile properties and regulate capillary blood flow. The aim of this study is to assess whether IAPP oligomers alter the morphology and contractility of pericytes using an in vitro brain vasculature model and evaluate the association between IAPP and capillary contraction in human brain tissue.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain Cell Line, Pericyte Cell Differentiation, Fibroblast

DISEASE(S): Type 2 Diabetes Mellitus

SUBMITTER: Charlotte Welinder

LAB HEAD: Malin Wennström

PROVIDER: PXD035048 | Pride | 2023-03-11

REPOSITORIES: Pride

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Contraction of human brain vascular pericytes in response to islet amyloid polypeptide is reversed by pramlintide.

Nuñez-Diaz Cristina C Pocevičiūtė Dovilė D Schultz Nina N Welinder Charlotte C Swärd Karl K Wennström Malin M

Molecular brain 20230215 1

The islet amyloid polypeptide (IAPP), a pancreas-produced peptide, has beneficial functions in its monomeric form. However, IAPP aggregates, related to type 2 diabetes mellitus (T2DM), are toxic not only for the pancreas, but also for the brain. In the latter, IAPP is often found in vessels, where it is highly toxic for pericytes, mural cells that have contractile properties and regulate capillary blood flow. In the current study, we use a microvasculature model, where human brain vascular peric ...[more]

PMID: 36793056

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