Proteomics

Dataset Information

0

Surface stiffness, hydration, polydimethylsiloxane (PDMS), hyaluronic acid, biofilm and bacterial mobility, protein patterns.


ABSTRACT: By a global proteomic approach and phenotypic assays, we investigated the impact of surface stiffness on E. coli biofilm formation capacity. A global proteomic approach was used in order to identify the most abundant proteins for the comparison between E. coli adhesion in PDMS 9 kPa and 574kPa, (polydimethylsiloxane,), and between HA 44Pa and 2kPa (hyaluronic acid gels).

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Escherichia Coli

SUBMITTER: Julie Hardouin  

LAB HEAD: Julie Hardouin

PROVIDER: PXD035233 | Pride | 2023-03-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
EColi_PDMS_1-5_1_02.raw Raw
EColi_PDMS_1-5_3_01.raw Raw
EColi_PDMS_1-5_4_01.raw Raw
EColi_PDMS_1-5_5_01.raw Raw
EColi_PDMS_1-80_1_01.raw Raw
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Publications

<i>Escherichia coli</i> Biofilm Formation, Motion and Protein Patterns on Hyaluronic Acid and Polydimethylsiloxane Depend on Surface Stiffness.

Vigué Annabelle A   Vautier Dominique D   Kaytoue Amad A   Senger Bernard B   Arntz Youri Y   Ball Vincent V   Ben Mlouka Amine A   Gribova Varvara V   Hajjar-Garreau Samar S   Hardouin Julie J   Jouenne Thierry T   Lavalle Philippe P   Ploux Lydie L  

Journal of functional biomaterials 20221111 4


The surface stiffness of the microenvironment is a mechanical signal regulating biofilm growth without the risks associated with the use of bioactive agents. However, the mechanisms determining the expansion or prevention of biofilm growth on soft and stiff substrates are largely unknown. To answer this question, we used PDMS (polydimethylsiloxane, 9-574 kPa) and HA (hyaluronic acid gels, 44 Pa-2 kPa) differing in their hydration. We showed that the softest HA inhibited Escherichia coli biofilm  ...[more]

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