Dataset Information

LRG1 is an adipokine that promotes insulin sensitivity and suppresses inflammation

ABSTRACT: While dysregulation of adipocyte endocrine function plays a central role in obesity and its complications, the vast majority of adipokines remain uncharacterized. We employed bio-orthogonal non-canonical amino acid tagging (BONCAT) and mass spectrometry to comprehensively characterize the secretome of murine visceral and subcutaneous white and interscapular brown adipocytes. Over 600 proteins were identified, the majority of which showed cell type-specific enrichment. We here describe a metabolic role for leucine-rich α-2 glycoprotein 1 (LRG1) as an obesity-regulated adipokine secreted by mature adipocytes. LRG1 overexpression significantly improved glucose homeostasis in diet-induced and genetically obese mice. This was associated with markedly reduced white adipose tissue macrophage accumulation and systemic inflammation. Mechanistically, we found LRG1 binds cytochrome c in circulation to dampen its pro-inflammatory effect. These data support a new role for LRG1 as an insulin sensitizer with therapeutic potential given its immunomodulatory function at the nexus of obesity, inflammation, and associated pathology.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Bile

SUBMITTER: henrik molina

LAB HEAD: Paul Cohen

PROVIDER: PXD035318 | Pride | 2022-11-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MS183737LUM_chan_cohen_control-AHA1_b.raw	Raw
	MS183737LUM_chan_cohen_control-AHA2_b.raw	Raw
	MS183737LUM_chan_cohen_control-AHA3_b.raw	Raw
	MS183737LUM_chan_cohen_control-PBS_b.raw	Raw
	MS183887LUM_chan_cohen_brown-AHA1_a.raw	Raw

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Publications

LRG1 is an adipokine that promotes insulin sensitivity and suppresses inflammation.

Choi Chan Hee J CHJ Barr William W Zaman Samir S Model Corey C Park Annsea A Koenen Mascha M Lin Zeran Z Szwed Sarah K SK Marchildon Francois F Crane Audrey A Carroll Thomas S TS Molina Henrik H Cohen Paul P

eLife 20221108

While dysregulation of adipocyte endocrine function plays a central role in obesity and its complications, the vast majority of adipokines remain uncharacterized. We employed bio-orthogonal non-canonical amino acid tagging (BONCAT) and mass spectrometry to comprehensively characterize the secretome of murine visceral and subcutaneous white and interscapular brown adip ocytes. Over 600 proteins were identified, the majority of which showed cell type-specific enrichment. We here describe a metabol ...[more]

PMID: 36346018

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