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Exit of spore dormancy transforms the yeast cytoplasm and the solubility of its proteome

ABSTRACT: The biophysical properties of the cytoplasm are a major determinant of key cellular processes. Yeasts produce dormant spores that can withstand extreme conditions. We show that spores exhibit extraordinary biophysical properties, including a highly viscous and acidic cytosol. These conditions alter the solubility of more than 100 proteins such as metabolic enzymes that become more soluble as spores transit to active cell proliferation upon nutrient repletion. A key regulator of this transition is the heat shock protein Hsp42, which shows transient solubilization and phosphorylation, and is essential for the transformation of the cytoplasm during germination. Germinating spores therefore return to growth through the dissolution of protein assemblies, orchestrated in part by Hsp42 activity. The modulation of spores’ molecular properties are likely key adaptive features of their exceptional survival capacities.

INSTRUMENT(S): Bruker Daltonics instrument model

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Jenny Moon

LAB HEAD: Christian R. Landry

PROVIDER: PXD035403 | Pride | 2023-03-14

REPOSITORIES: Pride

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			Action	DRS
	210413_orf_Y128_stat1_aa_cutoff.fasta	Fasta
	Landry_01_B9_1_1813.d.1.dar	Other
	Landry_02_C12_1_1864.d.1.dar	Other
	Landry_03_C8_1_1856.d.1.dar	Other
	Landry_04_D3_1_1872.d.1.dar	Other

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